| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RLN3 |
| Uniprot No | Q8WXF3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-52aa |
| 氨基酸序列 | RAAPY GVRLCGREFI RAVIFTCGGS RW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RLN3重组蛋白的3篇参考文献示例(内容基于学术研究主题模拟，非真实文献)：
---
1. **标题**：Recombinant Human RLN3 Production in E. coli: Optimization and Functional Characterization
**作者**：Smith A, et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达和纯化重组人RLN3蛋白的工艺优化，采用His标签和Ni-NTA层析技术。纯化后的蛋白通过圆二色谱证实其正确折叠，并在体外实验中激活RXFP3受体，诱导cAMP信号通路，为RLN3的功能研究提供可靠工具。
2. **标题**：RLN3 Recombinant Protein Modulates Stress Response in Rodent Hypothalamic Neurons
**作者**：Chen L, et al.
**摘要**：通过原代下丘脑神经元模型，研究发现重组RLN3蛋白显著抑制促肾上腺皮质激素释放激素(CRH)的分泌，并降低神经元兴奋性。该效应依赖RXFP3受体，表明RLN3可能通过重组蛋白干预应激相关神经通路，为治疗焦虑障碍提供新靶点。
3. **标题**：Structural Insights into RLN3-Receptor Interaction via Cryo-EM Analysis
**作者**：Wang Y, et al.
**摘要**：利用冷冻电镜技术解析重组RLN3蛋白与其受体RXFP3的复合物结构，揭示了RLN3特异性结合受体的关键氨基酸残基。该研究阐明了RLN3与松弛肽家族其他成员(如RLN1)的功能差异，为设计靶向RXFP3的激动剂奠定结构基础。
---
以上内容综合了RLN3重组蛋白的表达、功能及结构研究，涵盖生物工程、神经生物学和结构生物学领域，符合该蛋白的已知研究方向(如受体互作、应激调节)。
**Background of RLN3 Recombinant Protein**
Relaxin-3 (RLN3), a neuropeptide belonging to the relaxin/insulin superfamily, was first identified in 2002 due to its structural homology with relaxin-1 (RLN1) and relaxin-2 (RLN2). Encoded by the *RLN3* gene in humans, it is highly conserved across vertebrates, underscoring its evolutionary significance. RLN3 is predominantly expressed in the brain, specifically within the nucleus incertus, a region linked to stress responses, arousal, and memory.
Functionally, RLN3 acts as the endogenous ligand for the G protein-coupled receptor RXFP3 (relaxin family peptide 3 receptor). It also exhibits cross-reactivity with RXFP1. the receptor for RLN1 and RLN2. though with lower affinity. This dual-receptor interaction suggests roles in both neural and peripheral systems. RLN3 is implicated in modulating stress responses, appetite, circadian rhythms, and emotional behaviors, with animal studies highlighting its influence on anxiety, depression, and addiction pathways.
Recombinant RLN3 is produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and bioactivity. Its production enables detailed mechanistic studies, including receptor signaling, ligand-receptor binding dynamics, and *in vivo* functional assays. As a research tool, recombinant RLN3 aids in exploring its therapeutic potential for neuropsychiatric disorders, metabolic dysregulation, and neurodegenerative diseases. Challenges remain in optimizing receptor specificity and delivery methods, but RLN3 remains a promising target for drug development.
In summary, RLN3 recombinant protein serves as a critical reagent for unraveling the neurobiology of relaxin signaling and advancing translational applications.
×
