| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RXFP3 |
| Uniprot No | Q9NSD7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469aa |
| 氨基酸序列 | MQMADAATIATMNKAAGGDKLAELFSLVPDLLEAANTSGNASLQLPDLWWELGLELPDGAPPGHPPGSGGAESADTEARVRILISVVYWVVCALGLAGNLLVLYLMKSMQGWRKSSINLFVTNLALTDFQFVLTLPFWAVENALDFKWPFGKAMCKIVSMVTSMNMYASVFFLTAMSVTRYHSVASALKSHRTRGHGRGDCCGRSLGDSCCFSAKALCVWIWALAALASLPSAIFSTTVKVMGEELCLVRFPDKLLGRDRQFWLGLYHSQKVLLGFVLPLGIIILCYLLLVRFIADRRAAGTKGGAAVAGGRPTGASARRLSKVTKSVTIVVLSFFLCWLPNQALTTWSILIKFNAVPFSQEYFLCQVYAFPVSVCLAHSNSCLNPVLYCLVRREFRKALKSLLWRIASPSITSMRPFTATTKPEHEDQGLQAPAPPHAAAEPDLLYYPPGVVVYSGGRYDLLPSSSAY |
| 预测分子量 | 51,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RXFP3重组蛋白的3篇代表性文献摘要，供参考：
1. **文献名称**: "Recombinant RXFP3 relaxin receptors exhibit unique ligand binding characteristics"
**作者**: Smith CM, et al.
**摘要**: 该研究通过在大肠杆菌系统中重组表达并纯化RXFP3受体，发现其与内源性受体相比，对松弛素类似物具有更高的亲和力，提示重组蛋白在药物筛选中的潜在应用价值。
2. **文献名称**: "Structural insights into RXFP3 activation by peptide agonists"
**作者**: Liu Y, et al.
**摘要**: 利用冷冻电镜技术解析了重组RXFP3蛋白与不同配体结合的复合物结构，揭示了受体激活的构象变化机制，为靶向RXFP3的神经调节药物设计提供结构基础。
3. **文献名称**: "Functional characterization of RXFP3 signaling in recombinant cell models"
**作者**: van der Westhuizen ET, et al.
**摘要**: 在HEK293细胞中稳定表达重组RXFP3蛋白，证实其通过Gαi/o信号通路介导cAMP抑制，并发现受体脱敏现象与β-arrestin募集相关，为研究RXFP3生理功能提供体外模型。
注：以上文献信息为模拟生成，实际研究中建议通过PubMed/Google Scholar检索关键词"RXFP3 recombinant"获取最新数据。
RXFP3 (relaxin family peptide receptor 3) is a class A G protein-coupled receptor (GPCR) that primarily binds the neuropeptide relaxin-3 (RLN3). It plays critical roles in modulating stress responses, feeding behavior, circadian rhythms, and reproductive functions. Discovered in the early 2000s, RXFP3 is part of the relaxin receptor family, which includes RXFP1-4. but distinguishes itself through its high affinity for RLN3 and unique expression pattern in the brain, particularly in regions like the hypothalamus, amygdala, and nucleus accumbens.
Structurally, RXFP3 contains seven transmembrane domains characteristic of GPCRs, with a large extracellular N-terminal region involved in ligand binding. Signaling through RXFP3 primarily activates Gαi/o proteins, inhibiting cAMP production, but may also engage β-arrestin pathways, influencing neuronal excitability and synaptic plasticity. Its interaction with RLN3 is implicated in stress adaptation and energy homeostasis, making it a potential therapeutic target for anxiety, depression, obesity, and neurodegenerative disorders.
Recombinant RXFP3 proteins are engineered using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and membrane localization. These proteins often include affinity tags (e.g., His or FLAG) for purification and detection. Researchers utilize recombinant RXFP3 to study receptor-ligand dynamics, screen drug candidates, and resolve 3D structures via cryo-EM or crystallography, advancing mechanistic insights and drug discovery. Its role in neuroendocrine regulation continues to drive interest in both basic research and clinical applications.
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