| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC25 |
| Uniprot No | P30307 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKRCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP |
| 预测分子量 | 57.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于CDC25重组蛋白的参考文献摘要，按研究领域分类整理：
一、结构生物学研究
1. **文献名称**：Crystal Structure of Human CDC25B Phosphatase
**作者**：Reynolds RA, et al.
**摘要**：首次解析了人源CDC25B磷酸酶的晶体结构，揭示了其催化活性位点的构象特征，为设计靶向抑制剂提供结构基础。
二、重组表达与功能验证
2. **文献名称**：Expression and Purification of Active Recombinant CDC25C in E. coli
**作者**：Kristjánsdóttir K, et al.
**摘要**：开发了在大肠杆菌中高效表达可溶性CDC25C重组蛋白的方法，验证其体外磷酸酶活性及细胞周期调控功能。
三、药物筛选平台开发
3. **文献名称**：High-throughput Screening of CDC25 Inhibitors Using Recombinant Protein Assay
**作者**：Lavecchia A, et al.
**摘要**：基于重组CDC25A蛋白建立荧光底物检测体系，成功筛选出多个具有抗癌潜力的新型小分子抑制剂。
注：以上为领域代表性研究方向的模拟摘要，实际文献需通过PubMed/Google Scholar以"CDC25 recombinant protein"为关键词检索获取最新数据。近年研究热点集中于CDC25相分离机制(如Nature 2021)及变构调节(Cell Chem Biol 2022)等领域。
CDC25 recombinant proteins are derived from the CDC25 family of dual-specificity phosphatases, which play critical roles in regulating cell cycle progression. The CDC25 family consists of three isoforms in humans (CDC25A, CDC25B, and CDC25C) that activate cyclin-dependent kinase (CDK)/cyclin complexes by removing inhibitory phosphorylation marks. Specifically, they dephosphorylate conserved threonine and tyrosine residues on CDKs, enabling transitions through key cell cycle checkpoints. Dysregulation of CDC25 phosphatases is linked to uncontrolled proliferation, genomic instability, and cancer progression, making them subjects of interest in oncology research.
Recombinant CDC25 proteins are generated through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to study their enzymatic activity, structure, and interactions. These engineered proteins retain the catalytic core domain responsible for phosphatase activity while allowing scalable production for biochemical assays. Purification methods often involve affinity tags (e.g., GST or His-tags) to ensure high yield and purity.
In research, recombinant CDC25 proteins are utilized to:
1) Investigate cell cycle regulation mechanisms,
2) Screen small-molecule inhibitors for anticancer drug development,
3) Analyze substrate specificity through *in vitro* dephosphorylation assays,
4) Study mutations affecting phosphatase activity in disease models.
Their role in overriding cell cycle checkpoints has positioned CDC25 phosphatases as potential therapeutic targets. For example, CDC25B overexpression correlates with poor prognosis in multiple cancers, driving interest in developing inhibitors that block its interaction with CDK1/cyclin B1. However, isoform-specific functional redundancy and structural similarities pose challenges for targeted drug design, emphasizing the need for recombinant proteins to dissect isoform-specific biology.
Overall, CDC25 recombinant proteins serve as essential tools for unraveling cell cycle control pathways and advancing anticancer strategies.
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