| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC23 |
| Uniprot No | Q9UJX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-597aa |
| 氨基酸序列 | AAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYIKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLN |
| 预测分子量 | 71.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC23重组蛋白的3篇参考文献及其摘要概括：
1. **《Recombinant human CDC23 protein: Expression, purification, and functional analysis in the anaphase-promoting complex》**
- 作者：Li, X., et al.
- 摘要：研究通过大肠杆菌系统表达并纯化重组人源CDC23蛋白，验证其作为APC/C复合体核心亚基的功能，证实其参与泛素连接酶活性调控及细胞周期退出。
2. **《Structural insights into the role of CDC23 in APC/C assembly》**
- 作者：Yamaguchi, M., et al.
- 摘要：利用昆虫细胞表达系统获得重组CDC23蛋白，结合冷冻电镜解析其与APC/C其他亚基的相互作用，阐明CDC23在复合体组装中的关键结构作用。
3. **《Functional characterization of CDC23 mutations in yeast APC/C》**
- 作者：Thornton, B. R., et al.
- 摘要：通过酵母系统表达突变型CDC23重组蛋白，揭示其与APC/C底物识别及有丝分裂检查点调控的关联，为癌症相关突变提供机制解释。
注：上述文献为示例，实际研究中建议通过PubMed或Web of Science检索具体论文。CDC23作为APC/C复合体组分，相关研究多聚焦于其结构功能及细胞周期调控机制。
**Background of CDC23 Recombinant Protein**
CDC23 (Cell Division Cycle 23) is a conserved subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle regulation. The APC/C orchestrates the ubiquitination and subsequent degradation of key regulatory proteins, such as cyclins and securins, to drive transitions between cell cycle phases—particularly during mitosis and meiosis. CDC23. also known as APC8. plays a structural and functional role in maintaining APC/C stability and substrate recognition. It contains tetratricopeptide repeat (TPR) domains that mediate protein-protein interactions, enabling proper assembly of the APC/C complex and coordination with co-activators like CDC20 or CDH1.
Recombinant CDC23 proteins are engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional versions for biochemical and cellular studies. These proteins retain critical domains required for APC/C integration, making them valuable tools for dissecting mechanisms of cell cycle control, substrate specificity, and ubiquitination dynamics. Research applications include investigating mutations linked to mitotic errors, genomic instability, or cancer, where APC/C dysfunction is implicated. Additionally, recombinant CDC23 aids in drug discovery targeting cell cycle-related diseases. Its study also extends to developmental biology, as APC/C activity influences differentiation and tissue homeostasis. By enabling precise manipulation in vitro, recombinant CDC23 provides insights into fundamental processes governing cell division and their pathological disruptions.
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