| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASCL1 |
| Uniprot No | P50553 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-236aa |
| 氨基酸序列 | MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF |
| 预测分子量 | 29.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASCL1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *ASC1 reprograms lineage-committed cells into induced neurons*
**作者**: Wapinski, O. L., et al.
**摘要**: 本研究利用ASCL1重组蛋白联合其他转录因子，成功将成纤维细胞直接转分化为功能性神经元。实验表明，ASCL1通过激活神经元特异性基因并抑制非神经相关通路，驱动细胞命运转变。
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2. **文献名称**: *ASCL1 regulates pulmonary neuroendocrine cell differentiation via a transcriptional network*
**作者**: Borromeo, M. D., et al.
**摘要**: 通过表达ASCL1重组蛋白并结合染色质免疫沉淀分析，揭示了ASCL1在肺神经内分泌细胞分化中的核心作用，其通过结合特定DNA序列调控下游靶基因(如NEUROD1)的表达。
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3. **文献名称**: *Mechanistic insights into ASCL1-mediated somatic cell reprogramming*
**作者**: Park, N. J., et al.
**摘要**: 该研究探讨了ASCL1重组蛋白在体细胞重编程为诱导神经元中的分子机制，发现ASCL1通过招募染色质重塑复合物(如SWI/SNF)，促进神经元特异性增强子的激活。
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如需进一步文献，建议通过PubMed或Google Scholar以“ASCL1 recombinant protein”或“ASCL1 in vitro study”为关键词检索最新研究。
ASCL1 (Achaete-scute homolog 1), also known as MASH1. is a basic helix-loop-helix (bHLH) transcription factor critical in regulating cell fate determination and differentiation during embryonic development. It plays a pivotal role in neurogenesis and the development of endocrine cells, particularly in the nervous system and lungs. ASCL1 is essential for the specification of neuronal precursors, guiding the differentiation of neural stem cells into committed neuronal lineages by activating downstream targets like neuronal differentiation genes and repressing non-neuronal pathways.
Recombinant ASCL1 protein is engineered for research applications, typically produced in *E. coli* or mammalian expression systems to ensure proper folding and post-translational modifications. This purified protein retains the functional DNA-binding and dimerization domains, enabling studies on its interaction with E-box DNA motifs (CANNTG) and regulatory partners. Researchers use it to investigate mechanisms of neuronal reprogramming, as ASCL1 overexpression can convert fibroblasts or glial cells into induced neurons, offering potential in regenerative medicine.
In disease contexts, ASCL1 is implicated in neuroendocrine tumors (e.g., small cell lung cancer) and neurological disorders. Its dysregulation correlates with tumor aggressiveness, making it a biomarker and therapeutic target. Recombinant ASCL1 aids in screening small molecules that modulate its activity or in vitro modeling of developmental pathways. However, its proneural functions are context-dependent, sometimes suppressing metastasis in certain cancers. Current research focuses on balancing its pro-differentiation benefits against risks of oncogenic activation in therapeutic strategies.
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