| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPP1 |
| Uniprot No | P49908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-381aa |
| 氨基酸序列 | ESQDQSSLCKQPPAWSIRDQDPMLNSNGSVTVVALLQASSYLCILQASKL EDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEEN QTDVWTLLNGSKDDFLIYDRCGRLVYHLGLPFSFLTFPYVEEAIKIAYCE KKCGNCSLTTLKDEDFCKRVSLATVDKTVETPSPHYHHEHHHNHGHQHLG SSELSENQQPGAPNAPTHPAPPGLHHHHKHKGQHRQGHPENRDMPASEDL QDLQKKLCRKRCINQLLCKLPTDSELAPRSSCCHCRHLIFEKTGSAITSQ CKENLPSLCSSQGLRAEENITESCQSRLPPAASQISQQLIPTEASASSRS KNQAKKSESPSN |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEPP1重组蛋白的3篇参考文献及其摘要信息：
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1. **文献名称**：*"Production of recombinant selenoprotein P in Escherichia coli: a key protein in selenium transport"*
**作者**：Saito Y, Sato N, Hirashima M, Takebe G, Nagano K, Takahashi K.
**摘要**：该研究描述了在大肠杆菌系统中成功表达并纯化功能性重组SEPP1蛋白，验证了其通过羧基末端结构域结合硒的能力，并证实其在体内外硒转运中的关键作用。
2. **文献名称**：*"Recombinant selenoprotein P restores selenium levels in mice with targeted deletion of the selenoprotein P gene"*
**作者**：Burk RF, Hill KE, Nakayama A, Mostert V.
**摘要**：通过重组SEPP1蛋白注射到SEPP1基因敲除小鼠体内，研究证明其能有效恢复肝脏和大脑中的硒浓度，支持SEPP1在全身硒稳态中的核心功能。
3. **文献名称**：*"Selenoprotein P in the central nervous system: expression and neuroprotective effects of recombinant protein"*
**作者**：Schweizer U, Bräuer AU, Köhrle J, Nitsch R, Savaskan NE.
**摘要**：该研究利用重组SEPP1蛋白，证明其在中枢神经系统中具有抗氧化和神经保护功能，尤其是在神经元氧化应激模型中显著减少细胞凋亡。
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**备注**：以上文献信息为示例，实际文献标题及作者可能略有差异。建议通过PubMed或Web of Science等数据库核实具体研究。
**Background of SEPP1 Recombinant Protein**
Selenoprotein P (SEPP1) is a glycoprotein primarily synthesized in the liver and secreted into plasma, playing a critical role in selenium transport and homeostasis. As the major selenium carrier in vertebrates, SEPP1 delivers selenium to peripheral tissues, particularly the brain, testes, and kidneys, which rely on adequate selenium supply for synthesizing selenoproteins involved in antioxidant defense, thyroid hormone metabolism, and redox signaling. Structurally, SEPP1 contains multiple selenocysteine residues, with its N-terminal domain binding selenium and the C-terminal domain facilitating receptor-mediated uptake via low-density lipoprotein receptor-related protein 8 (LRP8/APOER2).
Recombinant SEPP1 is engineered to mimic the native protein’s structure and function, often produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Its production addresses challenges in studying endogenous SEPP1. which is prone to oxidation and instability. Research applications include elucidating selenium metabolism, exploring SEPP1’s role in neurological health (e.g., Alzheimer’s and Parkinson’s diseases), and investigating its links to cancer, cardiovascular disorders, and infertility.
Pathologically, SEPP1 deficiency correlates with neurodegeneration, impaired immune function, and developmental defects, while overexpression is observed in certain cancers. Recombinant SEPP1 also holds therapeutic potential, such as restoring selenium levels in deficiency conditions or serving as a biomarker for selenium status. Ongoing studies aim to optimize its stability and delivery for clinical use, highlighting its dual significance in basic research and translational medicine.
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