关键信息
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基因名
ROS1
- 应用
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别名
ROS1；MCF3；ROS；Proto-oncogene tyrosine-Protein kinase ROS
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08922
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表达区间
1881-2347aa
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氨基酸序列
VWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPT QEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVK TLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELME GRDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDL AARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAP ESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRL EPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKSR DEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATE CGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEKQVAYCPSGKPE GLNYACLTHSGYGDGSD
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分子量
82 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Research on recombinant ROS1 (Receptor of Activated C Kinase 1) proteins has gained prominence due to their critical roles in various biological processes and disease pathways, particularly in cancer. ROS1 is a receptor tyrosine kinase that, when rearranged or mutated, has been implicated in the pathogenesis of several tumors, notably non-small cell lung cancer (NSCLC). Understanding the structural and functional characteristics of ROS1 is crucial for the development of targeted therapies. With advancements in recombinant DNA technology, scientists can produce large quantities of pure ROS1 proteins, enabling detailed studies of their signaling mechanisms and interactions with other cellular proteins. This research facilitates the exploration of novel therapeutic strategies, including small-molecule inhibitors and monoclonal antibodies against aberrant ROS1 pathways. Additionally, studying recombinant ROS1 aids in the comprehension of its normal biological functions in cell proliferation and differentiation, thereby providing insights into potential clinical applications. The therapeutic potential of targeting ROS1 in oncology underscores the necessity for continued investigation into recombinant forms of the protein, as they hold promise for improving patient outcomes in ROS1-positive malignancies.
