| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF596 |
| Uniprot No | Q8TC21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-498 aa |
| 活性数据 | MTFEDIIVDFTQEEWALLDTSQRKLFQDVMLENISHLVSIGKQLCKSVVLSQLEQVEKLSTQRISLLQGREVGIKHQEIPFIHHIYQKGTSTISTMRSHTQEDPFLCNDLGEDFTQHIALTQNVITYMRTKHFVSKKFGKIFSDWLSFNQHKEIHTKCKSYGSHLFDYAFIQNSALRPHSVTHTREITLECRVCGKTFSKNSNLRRHEMIHTGEKPHGCHLCGKAFTHCSDLRKHERTHTGEKPYGCHLCGKAFSKSSNLRRHEMIHTREKAQICHLCGKAFTHCSDLRKHERTHLGDKPYGCLLCGKAFSKCSYLRQHERTHNGEKPYECHLCGKAFSHCSHLRQHERSHNGEKPHGCHLCGKAFTESSVLKRHERIHTGEKPYECHVCGKAFTESSDLRRHERTHTGEKPYECHLCGKAFNHSSVLRRHERTHTGEKPYECNICGKAFNRSYNFRLHRRVHTGEKPYVCPLCGKAFSKFFNLRQHERTHTKKAMNM |
| 分子量 | 84.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人ZNF596蛋白的文献示例(内容基于虚构概括，实际文献需通过学术数据库查询)：
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1. **文献名称**: *ZNF596 as a novel transcriptional repressor in neuronal differentiation*
**作者**: Li X, et al.
**摘要**: 研究发现ZNF596通过其C2H2型锌指结构域与特定DNA序列结合，抑制神经分化相关基因的表达，在小鼠胚胎干细胞分化模型中调控神经元发育。
2. **文献名称**: *Structural and functional analysis of ZNF596 in DNA damage response*
**作者**: Wang Y, et al.
**摘要**: 揭示ZNF596通过参与DNA损伤修复通路，与BRCA1蛋白相互作用，维持基因组稳定性，其重组蛋白的过表达可增强细胞对电离辐射的抗性。
3. **文献名称**: *ZNF596 interaction with lncRNAs in cancer progression*
**作者**: Kim S, et al.
**摘要**: 提出ZNF596在胃癌中异常高表达，与长链非编码RNA(如HOTAIR)结合，促进肿瘤细胞增殖和转移，或成为潜在治疗靶点。
4. **文献名称**: *Zinc finger domain mutagenesis of ZNF596 alters chromatin binding*
**作者**: Gomez F, et al.
**摘要**: 通过点突变实验验证ZNF596锌指结构域中关键氨基酸残基对其染色质定位及基因沉默功能的影响，为蛋白工程改造提供依据。
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**提示**：若需真实文献，建议在PubMed、Google Scholar等平台以“ZNF596 protein”或“recombinant ZNF596”为关键词检索，重点关注功能机制、结构解析或疾病关联研究。
ZNF596. a member of the zinc finger protein family, is a transcription factor characterized by its C2H2-type zinc finger domains, which facilitate sequence-specific DNA binding. Predominantly expressed in human tissues, it is implicated in regulating gene expression by interacting with target DNA sequences or partnering with other proteins. Studies suggest its involvement in cellular processes like differentiation, proliferation, and apoptosis. ZNF596 may act as a tumor suppressor or oncogene depending on context, with dysregulation linked to cancers, though its precise mechanisms remain under investigation.
Recombinant human ZNF596 protein is engineered via molecular cloning, often expressed in systems like *E. coli* or mammalian cells for functional studies. Its recombinant form enables biochemical assays (e.g., EMSA, ChIP) to map DNA-binding sites, analyze protein interactions, or explore post-translational modifications. Researchers also utilize it to screen for therapeutic compounds targeting ZNF596-associated pathways. Despite progress, its full regulatory network and tissue-specific roles require further elucidation, particularly in neurological disorders and cancer progression. Overall, recombinant ZNF596 serves as a critical tool for deciphering its biological significance and therapeutic potential.
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