| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF589 |
| Uniprot No | Q86UQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364 aa |
| 活性数据 | MWAPREQLLGWTAEALPAKDSAWPWEEKPRYLGPVTFEDVAVLFTEAEWKRLSLEQRNLYKEVMLENLRNLVSLAESKPEVHTCPSCPLAFGSQQFLSQDELHNHPIPGFHAGNQLHPGNPCPEDQPQSQHPSDKNHRGAEAEDQRVEGGVRPLFWSTNERGALVGFSSLFQRPPISSWGGNRILEIQLSPAQNASSEEVDRISKRAETPGFGAVTFGECALAFNQKSNLFRQKAVTAEKSSDKRQSQVCRECGRGFSRKSQLIIHQRTHTGEKPYVCGECGRGFIVESVLRNHLSTHSGEKPYVCSHCGRGFSCKPYLIRHQRTHTREKSFMCTVCGRGFREKSELIKHQRIHTGDKPYVCRD |
| 分子量 | 40.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF589蛋白的模拟参考文献示例(基于公开领域知识推断，非真实文献)：
1. **文献名称**：*ZNF589 regulates stem cell pluripotency through histone modification*
**作者**：Chen L, et al.
**摘要**：研究利用重组ZNF589蛋白揭示了其通过招募组蛋白脱乙酰酶(HDACs)抑制多能性基因表达，从而调控胚胎干细胞分化。
2. **文献名称**：*Structural characterization of recombinant human ZNF589 and its DNA-binding specificity*
**作者**：Wang Y, et al.
**摘要**：通过大肠杆菌表达重组ZNF589蛋白并解析其结构，证明其锌指结构域选择性结合富含GC的DNA序列，为功能研究提供基础。
3. **文献名称**：*ZNF589 suppresses tumor metastasis by modulating epithelial-mesenchymal transition in lung cancer*
**作者**：Kimura T, et al.
**摘要**：体外实验表明，重组ZNF589过表达可抑制肺癌细胞EMT进程，其机制可能与抑制TGF-β信号通路相关。
4. **文献名称**：*High-throughput screening identifies ZNF589 as a novel biomarker for Alzheimer’s disease*
**作者**：Rodriguez S, et al.
**摘要**：基于重组ZNF589蛋白的蛋白质组学分析发现，其在阿尔茨海默病患者脑脊液中表达异常，提示可能参与tau蛋白病理过程。
**备注**：上述文献为基于ZNF蛋白家族典型研究方向的模拟内容，实际研究需参考真实数据库(如PubMed)。
ZNF589 (Zinc Finger Protein 589) is a member of the Kruppel-associated box (KRAB) domain-containing zinc finger protein family, which represents one of the largest groups of transcription factors in the human genome. These proteins are characterized by tandem arrays of C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, coupled with a KRAB domain at the N-terminus known to recruit transcriptional repressor complexes. ZNF589 is implicated in transcriptional regulation, chromatin remodeling, and epigenetic silencing, though its precise biological functions remain less explored compared to other zinc finger proteins.
Recombinant human ZNF589 protein is typically produced using heterologous expression systems, such as *E. coli*, yeast, or mammalian cell lines, to enable structural and functional studies. The recombinant protein allows researchers to investigate its DNA-binding specificity, interactions with co-repressors (e.g., TRIM28/KAP1), and role in gene regulatory networks. Its KRAB domain is of particular interest for engineered CRISPR/dCas9 systems, where it can be repurposed for targeted gene silencing.
Despite its potential, ZNF589's physiological relevance is still emerging. Limited studies suggest associations with cancer progression, neuronal development, and immune responses. Structural insights from recombinant protein studies may clarify its mechanisms and disease links, paving the way for therapeutic applications in epigenetics or synthetic biology. Further research is needed to map its target genes and regulatory pathways comprehensively.
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