| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF585A |
| Uniprot No | Q6P3V2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-113 aa |
| 活性数据 | MPANWTSPQKSSALAPEDHGSSYEGSVSFRDVAIDFSREEWRHLDPSQRNLYRDVMLETYSHLLSIGYQVPEAEVVMLEQGKEPWALQGERPRQSCPAPCLVNSHHLQESFRG |
| 分子量 | 39.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF585A蛋白的假设性参考文献示例(注：基于当前公开信息，ZNF585A研究可能有限，以下为模拟生成内容供参考)：
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1. **文献名称**：*"Functional characterization of recombinant ZNF585A as a transcriptional repressor in human cells"*
**作者**：Chen L, et al.
**摘要**：本研究通过原核表达系统成功纯化重组人ZNF585A蛋白，并证实其通过特异性结合DNA启动子区域抑制靶基因(如癌症相关基因)的转录活性，揭示了其在表观遗传调控中的潜在作用。
2. **文献名称**：*"Structural analysis of zinc finger domain in ZNF585A and its interaction with nucleic acids"*
**作者**：Zhang Y, Wang R.
**摘要**：利用X射线晶体学解析了ZNF585A蛋白的锌指结构域三维结构，结合体外实验证明其优先结合富含GC碱基的DNA序列，为理解该蛋白在基因调控中的分子机制提供了结构基础。
3. **文献名称**：*"Expression and purification of recombinant human ZNF585A in HEK293 cells for functional studies"*
**作者**：Kim S, et al.
**摘要**：优化了ZNF585A在哺乳动物细胞中的重组表达条件，获得了高纯度蛋白，并通过ChIP-seq技术鉴定了其基因组结合位点，提示其在细胞分化和发育过程中的调控功能。
4. **文献名称**：*"Association of ZNF585A polymorphisms with autoimmune diseases and its protein expression profiling"*
**作者**：Gupta M, et al.
**摘要**：发现ZNF585A基因多态性与系统性红斑狼疮(SLE)风险相关，并通过ELISA检测患者血清中重组ZNF585A蛋白水平异常，提示其可能参与自身免疫反应的调控通路。
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**注意**：以上文献为模拟生成，实际研究中需根据具体数据库(如PubMed、Web of Science)检索核实。若研究较少，建议扩展至ZNF蛋白家族或相关通路文献。
**Background of Recombinant Human ZNF585A Protein**
Zinc finger protein 585A (ZNF585A) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2) zinc finger domains that mediate sequence-specific DNA or RNA binding. This protein is hypothesized to function as a transcription regulator, potentially influencing gene expression patterns during development, cellular differentiation, or stress responses. ZNF585A is encoded by a primate-specific gene, suggesting its role may be linked to evolutionary adaptations in higher organisms. Although its precise biological mechanisms remain understudied, structural predictions indicate involvement in chromatin remodeling or epigenetic regulation, possibly through interactions with DNA methyltransferases or histone modifiers.
Recombinant human ZNF585A protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Purified recombinant ZNF585A facilitates *in vitro* analyses, such as DNA-binding assays, protein-protein interaction screens, or structural studies (e.g., X-ray crystallography). Research highlights its potential association with neurodevelopmental disorders, cancers, and cardiovascular diseases, where dysregulation of zinc finger proteins often correlates with pathogenic gene expression profiles. For instance, ZNF585A expression variations have been observed in tumor tissues, implying a possible role in oncogenesis or metastasis suppression.
Current studies aim to unravel its target genes, regulatory networks, and post-translational modifications, which could advance its utility as a biomarker or therapeutic target. Generating recombinant ZNF585A remains critical for dissecting its molecular functions and disease relevance.
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