| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF563 |
| Uniprot No | Q8TA94 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476 aa |
| 活性数据 | MDAVAFEDVAVNFTQEEWALLGPSQKNLYRYVMQETIRNLDCIRMIWEEQNTEDQYKNPRRNLRCHMVERFSESKDSSQCGETFSLIRDSIVNNSICPGEDPCQSAECEEVIMGHLSLNSHIRVDSGHKPHEYQEYGEKPHTHKQRGKAFSYHHSFQSRGRPHTGKKRYECKECGKTFSSRRNLRRHMVVQGGNRPYKCKLCGKAFFWPSLLRMHERTHTGEKPYECKQCSKAFPFYSSYRRHERMHTGEKPYECKQCSKALPDSSSYIRHERTHTGEKPYTCKQCGKAFSVSSSLRRHETTHSAEKPYECKQCGKTFHHLGSFQIHMKRHTGDRPHKCKICGKGFDRPSLVRYHERIHTGEKPYECKQCGKTLSHSSSFRRHMIMHTGGGPHKCKICGKAFVYPSVCQRHEKSHSGEKPYECKQCGKALSHSSSFRRHMVMHTGDGPNKCKVCGKAFVYPSVCQRHEKTHWRETI |
| 分子量 | 81.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于重组人ZNF563蛋白的参考文献示例(实际文献需根据具体数据库检索确认)：
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1. **文献名称**：*Functional Characterization of Recombinant Human ZNF563 as a Transcriptional Repressor in Cell Cycle Regulation*
**作者**：Zhang Y. et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化重组人ZNF563蛋白，通过染色质免疫沉淀(ChIP)和报告基因实验证实其通过结合特定DNA序列抑制细胞周期相关基因转录，揭示其在肿瘤发生中的潜在作用。
2. **文献名称**：*Structural Insights into the Zinc Finger Domains of Human ZNF563 by Recombinant Protein Analysis*
**作者**：Lee S., Kim J.H.
**摘要**：通过X射线晶体学解析重组ZNF563蛋白的锌指结构域三维结构，揭示其与DNA结合的分子机制，为设计靶向锌指蛋白的小分子药物提供结构基础。
3. **文献名称**：*Recombinant ZNF563 Protein Enhances Neuronal Differentiation in Stem Cell Models*
**作者**：Müller P. et al.
**摘要**：在哺乳动物细胞中表达重组ZNF563蛋白，发现其通过激活神经分化关键通路(如Wnt/β-catenin)促进胚胎干细胞向神经元分化，暗示其在神经再生治疗中的应用潜力。
4. **文献名称**：*High-Yield Production of Recombinant ZNF563 in Insect Cells and Its Role in Apoptosis Regulation*
**作者**：Gupta R., Wong L.
**摘要**：利用杆状病毒-昆虫细胞系统实现ZNF563的高效表达，纯化后蛋白功能实验表明其通过调控Bcl-2家族蛋白抑制癌细胞凋亡，提示其作为癌症治疗靶点的可能性。
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**注意**：以上内容为示例，实际文献需通过PubMed、Google Scholar等平台以“ZNF563”及“recombinant protein”为关键词检索获取。若研究较少，可扩展检索范围至锌指蛋白家族或相关功能通路。
Zinc finger protein 563 (ZNF563) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KZNF) family, which represents the largest subgroup of transcription factors in humans. These proteins are characterized by tandem Cys2-His2 (C2H2) zinc finger motifs that mediate sequence-specific DNA binding, coupled with a KRAB domain at the N-terminus implicated in transcriptional repression via interaction with co-repressors. ZNF563 is encoded by a gene located on chromosome 19 (19q13.43) and is hypothesized to regulate gene expression during development, cellular differentiation, or stress responses, though its precise biological functions remain incompletely understood.
Studies suggest ZNF563 may play roles in modulating cell proliferation, apoptosis, or tumorigenesis. For instance, aberrant expression has been observed in certain cancers, including hepatocellular carcinoma and glioblastoma, implying potential involvement in oncogenic pathways. As a recombinant protein, ZNF563 is typically produced in vitro using expression systems (e.g., *E. coli* or mammalian cells) to study its DNA-binding specificity, interactome, or regulatory mechanisms. Its recombinant form enables structural analyses (e.g., crystallography) and functional assays to dissect its role in chromatin remodeling or epigenetic regulation.
Current research focuses on elucidating ZNF563's target genes, signaling networks, and therapeutic relevance, particularly in diseases linked to transcriptional dysregulation. Advances may contribute to diagnostic or therapeutic strategies for cancer or genetic disorders.
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