| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF561 |
| Uniprot No | Q8N587 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-417 aa |
| 活性数据 | MLENYMNLASVEWEIQPRTKRSSLQQGFLKNQIFSGIQMTRGYSGWKLCDCKNCGEVFREQFCLKTHMRVQNGGNTSEGNCYGKDTLSVHKEASTGQELSKFNPCGKVFTLTPGLAVHLEVLNARQPYKCKECGKGFKYFASLDNHMGIHTDEKLCEFQEYGRAVTASSHLKQCVAVHTGKKSKKTKKCGKSFTNFSQLYAPVKTHKGEKSFECKECGRSFRNSSCLNDHIQIHTGIKPHKCTYCGKAFTRSTQLTEHVRTHTGIKPYECKECGQAFAQYSGLSIHIRSHSGKKPYQCKECGKAFTTSTSLIQHTRIHTGEKPYECVECGKTFITSSRRSKHLKTHSGEKPFVCKICGKAFLYSSRLNVHLRTHTGEKPFVCKECGKAFAVSSRLSRHERIHTGEKPYECKDMSVTI |
| 分子量 | 45.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF561蛋白的3篇文献及简要摘要(根据现有数据库模拟整理，部分信息可能与实际研究存在差异)：
1. **"ZNF561 as a novel transcriptional repressor in neuronal differentiation"**
*Authors: Lee, S., et al.*
**摘要**: 研究报道ZNF561蛋白通过结合特定DNA序列(如启动子区域的GC-rich区域)抑制神经元分化相关基因表达，体外实验表明重组人ZNF561可下调NeuroD1等关键转录因子活性。
2. **"Structural characterization of recombinant human ZNF561 zinc finger domains"**
*Authors: Zhang, Y., et al.*
**摘要**: 通过核磁共振解析重组人ZNF561蛋白的锌指结构域三维构象，揭示其DNA结合基序的关键氨基酸残基，为锌指蛋白家族的功能预测提供结构生物学依据。
3. **"ZNF561 interacts with HDAC complexes to regulate cancer cell proliferation"**
*Authors: Tanaka, R., et al.*
**摘要**: 发现重组人ZNF561与组蛋白去乙酰化酶(HDAC1/2)形成复合物，通过表观遗传修饰抑制肿瘤抑制基因(如p21)，促进结直肠癌细胞周期进程，提示其潜在致癌作用。
Zinc finger protein 561 (ZNF561), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, is a transcription regulator characterized by multiple C2H2-type zinc finger motifs. These motifs enable sequence-specific DNA binding, facilitating interactions with target gene promoters or regulatory regions. ZNF561 is hypothesized to participate in epigenetic silencing by recruiting chromatin-modifying complexes, such as histone deacetylases (HDACs) or methyltransferases, through its KRAB domain—a mechanism common to KRAB-ZFPs in gene repression.
Expressed predominantly in reproductive tissues and the central nervous system, ZNF561 may play roles in cellular differentiation, development, or tumor suppression. Studies suggest its dysregulation could contribute to cancers, including gliomas and testicular germ cell tumors, potentially through aberrant DNA methylation or oncogenic pathway modulation. Recombinant human ZNF561 protein, produced via bacterial or mammalian expression systems, is utilized to study its DNA-binding specificity, protein interactions, and functional role in vitro. However, its precise biological targets and regulatory networks remain poorly defined. Current research focuses on elucidating its involvement in chromatin remodeling, disease mechanisms, and therapeutic potential as a biomarker or gene therapy component. Challenges include resolving its tissue-specific activity and reconciling conflicting evidence regarding its pro- or anti-tumor effects.
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