| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF447 |
| Uniprot No | Q8TBC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-510 aa |
| 活性数据 | MLPLEKAFASPRSSPAPPDLPTPGSAAGVQQEEPETIPERTPADLEFSRLRFREFVYQEAAGPHQTLARLHELCRQWLMPEARSKEQMLELLVLEQFLGILPDKVRPWVVAQYPESCKKAASLVEGLADVLEEPGMLLGSPAGSSSILSDGVYERHMDPLLLPGELASPSQALGAGEIPAPSETPWLSPDPLFLEQRRVREAKTEEDGPANTEQKLKSFPEDPQHLGEWGHLDPAEENLKSYRKLLLWGYQLSQPDAASRLDTEELRLVERDPQGSSLPEGGRRQESAGCACEEAAPAGVLPELPTEAPPGDALADPPSGTTEEEEEQPGKAPDPQDPQDAESDSATGSQRQSVIQQPAPDRGTAKLGTKRPHPEDGDEQSLEGVSSSGDSAGLEAGQGPGADEPGLSRGKPYACGECGEAFAWLSHLMEHHSSHGGRKRYACQGCWKTFHFSLALAEHQKTHEKEKSYALGGARGPQPSTREAQAGARAGGPPESVEGEAPPAPPEAQR |
| 分子量 | 81.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性示例文献(非真实存在，供参考格式及内容方向)：
1. **文献名称**: "ZNF447 as a Transcriptional Repressor in DNA Methylation Regulation"
**作者**: Smith J, et al.
**摘要**: 研究证实ZNF447通过与DNA甲基转移酶相互作用，在基因启动子区域招募甲基化修饰，调控胚胎干细胞分化过程中的基因沉默。
2. **文献名称**: "Structural Characterization of Recombinant ZNF447 Protein"
**作者**: Lee S, et al.
**摘要**: 首次报道重组人ZNF447蛋白的原核表达及纯化方法，通过X射线晶体学解析其C2H2锌指结构域的三维构象，揭示其DNA结合机制。
3. **文献名称**: "ZNF447 Modulates Wnt/β-catenin Signaling in Cancer Metastasis"
**作者**: Wang X, et al.
**摘要**: 证明ZNF447通过结合β-catenin抑制Wnt通路活性，重组ZNF447蛋白的过表达可减少肿瘤细胞侵袭，提示其作为潜在抑癌因子。
4. **文献名称**: "Functional Redundancy of ZNF447 and ZNF469 in Genomic Stability"
**作者**: Tanaka R, et al.
**摘要**: 利用CRISPR技术构建ZNF447敲除细胞系，发现其与ZNF469协同维持端粒重复序列稳定性，重组蛋白回补实验验证功能。
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**备注**：上述文献为模拟示例，实际研究中建议通过PubMed、Google Scholar等平台以“ZNF447 recombinant protein”、“ZNF447 function”等关键词检索最新文献。真实研究方向可能涵盖表观遗传调控、癌症生物学或发育生物学领域。
Zinc finger protein 447 (ZNF447) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2)-type zinc finger domains that facilitate DNA or RNA binding. Primarily localized in the nucleus, ZNF447 is implicated in transcriptional regulation, though its precise biological roles remain incompletely understood. Studies suggest its involvement in chromatin remodeling, epigenetic modifications, and cell differentiation processes. The protein contains a KRAB (Krüppel-associated box) domain, commonly associated with transcriptional repression through interactions with chromatin-modifying complexes. ZNF447 is evolutionarily conserved across vertebrates, indicating potential roles in essential developmental pathways.
Emerging evidence links ZNF447 to cancer biology, where it may act as either an oncogene or tumor suppressor depending on cellular context. For instance, it has been reported to suppress certain viral promoters and modulate retrovirus silencing in embryonic stem cells. Recombinant human ZNF447 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies to dissect its molecular interactions and regulatory mechanisms. Despite progress, the full spectrum of its targets, partners, and disease associations requires further exploration. Current research focuses on elucidating its role in stem cell pluripotency, genome stability, and potential therapeutic applications in malignancies or genetic disorders.
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