| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFP161 |
| Uniprot No | O43829 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-449 aa |
| 活性数据 | MEFFISMSETIKYNDDDHKTLFLKTLNEQRLEGEFCDIAIVVEDVKFRAHRCVLAACSTYFKKLFKKLEVDSSSVIEIDFLRSDIFEEVLNYMYTAKISVKKEDVNLMMSSGQILGIRFLDKLCSQKRDVSSPDENNGQSKSKYCLKINRPIGDAADTQDDDVEEIGDQDDSPSDDTVEGTPPSQEDGKSPTTTLRVQEAILKELGSEEVRKVNCYGQEVESMETPESKDLGSQTPQALTFNDGMSEVKDEQTPGWTTAASDMKFEYLLYGHHREQIACQACGKTFSDEGRLRKHEKLHTADRPFVCEMCTKGFTTQAHLKEHLKIHTGYKPYSCEVCGKSFIRAPDLKKHERVHSNERPFACHMCDKAFKHKSHLKDHERRHRGEKPFVCGSCTKAFAKASDLKRHENNMHSERKQVTPSAIQSETEQLQAAAMAAEAEQQLETIACS |
| 分子量 | 77.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人ZFP161蛋白相关的参考文献摘要(基于公开数据虚构，实际文献需通过学术数据库检索确认)：
1. **《ZFP161 regulates cell proliferation through TGF-β/Smad signaling pathway》**
- 作者：Li, X. et al.
- 摘要：研究报道了重组人ZFP161蛋白在HEK293细胞中的表达纯化，并发现其通过结合Smad7启动子区抑制TGF-β信号通路，进而影响肿瘤细胞增殖和迁移。
2. **《Structural characterization of recombinant human ZFP161 and its DNA-binding specificity》**
- 作者：Zhang, Y. et al.
- 摘要：利用X射线晶体学解析了重组ZFP161蛋白的锌指结构域，揭示其与靶DNA序列5'-GCCATC-3'的特异性结合模式，为基因编辑工具开发提供结构基础。
3. **《ZFP161 interacts with HDAC1 to suppress hepatocellular carcinoma metastasis》**
- 作者：Wang, H. et al.
- 摘要：通过免疫共沉淀实验证实重组ZFP161蛋白与组蛋白去乙酰化酶HDAC1存在物理相互作用，协同抑制肝细胞癌中MMP9等转移相关基因的转录。
提示：实际研究中建议通过PubMed或Web of Science以"ZFP161"、"recombinant ZFP161"为关键词检索最新文献，并优先选择近五年内发表的论文。
ZFP161. also known as Zinc Finger Protein 161 or JAZ, is a member of the C2H2-type zinc finger protein family characterized by conserved cysteine and histidine residues that coordinate zinc ions to stabilize its structure. This nuclear protein contains an N-terminal POZ/BTB domain, which mediates protein-protein interactions, and multiple C-terminal zinc finger motifs responsible for sequence-specific DNA binding. Initially identified for its role in transcriptional regulation, ZFP161 acts as a transcriptional repressor by recruiting corepressors like NCoR and HDACs through its POZ domain. It interacts with tumor suppressor RB1 and modulates cell cycle progression, apoptosis, and differentiation.
Studies link ZFP161 to cancer biology, where it exhibits dual roles depending on cellular context. In some cancers, it suppresses oncogenes (e.g., MYC) and inhibits tumor growth, while in others, it may promote invasiveness by regulating matrix metalloproteinases. Its involvement in embryonic development and stem cell maintenance has also been observed. Recombinant human ZFP161 protein, typically produced via bacterial or mammalian expression systems, is utilized to study its DNA-binding properties, protein interactome, and mechanisms in disease models. Current research explores its therapeutic potential as a transcriptional modulator or a biomarker in precision oncology.
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