| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZER1 |
| Uniprot No | Q7Z7L7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-766 aa |
| 活性数据 | MASDTPESLMALCTDFCLRNLDGTLGYLLDKETLRLHPDIFLPSEICDRLVNEYVELVNAACNFEPHESFFSLFSDPRSTRLTRIHLREDLVQDQDLEAIRKQDLVELYLTNCEKLSAKSLQTLRSFSHTLVSLSLFGCTNIFYEEENPGGCEDEYLVNPTCQVLVKDFTFEGFSRLRFLNLGRMIDWVPVESLLRPLNSLAALDLSGIQTSDAAFLTQWKDSLVSLVLYNMDLSDDHIRVIVQLHKLRHLDISRDRLSSYYKFKLTREVLSLFVQKLGNLMSLDISGHMILENCSISKMEEEAGQTSIEPSKSSIIPFRALKRPLQFLGLFENSLCRLTHIPAYKVSGDKNEEQVLNAIEAYTEHRPEITSRAINLLFDIARIERCNQLLRALKLVITALKCHKYDRNIQVTGSAALFYLTNSEYRSEQSVKLRRQVIQVVLNGMESYQEVTVQRNCCLTLCNFSIPEELEFQYRRVNELLLSILNPTRQDESIQRIAVHLCNALVCQVDNDHKEAVGKMGFVVTMLKLIQKKLLDKTCDQVMEFSWSALWNITDETPDNCEMFLNFNGMKLFLDCLKEFPEKQELHRNMLGLLGNVAEVKELRPQLMTSQFISVFSNLLESKADGIEVSYNACGVLSHIMFDGPEAWGVCEPQREEVEERMWAAIQSWDINSRRNINYRSFEPILRLLPQGISPVSQHWATWALYNLVSVYPDKYCPLLIKEGGMPLLRDIIKMATARQETKEMARKVIEHCSNFKEENMDTSR |
| 分子量 | 114.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZER1蛋白的3篇代表性文献(注：ZER1相关研究较少，以下为模拟示例)：
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1. **文献名称**：ZER1 regulates epithelial-mesenchymal transition by modulating E-cadherin expression.
**作者**：Smith A, et al.
**摘要**：本研究揭示了ZER1蛋白通过调控E-cadherin转录参与上皮-间充质转化(EMT)。利用重组人ZER1蛋白进行体外结合实验，证明其直接与E-cadherin启动子区域的特定序列相互作用，抑制肿瘤细胞迁移。
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2. **文献名称**：Structural and functional characterization of recombinant human ZER1 in cell cycle progression.
**作者**：Li X, et al.
**摘要**：作者通过大肠杆菌系统表达并纯化了重组人ZER1蛋白，解析了其晶体结构。功能实验表明，ZER1通过结合Cyclin B1调控G2/M期转换，敲低ZER1导致细胞周期停滞。
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3. **文献名称**：ZER1 interacts with the ubiquitin ligase complex in protein quality control.
**作者**：Wang Y, et al.
**摘要**：本文证明重组ZER1蛋白在体外与CUL4-DDB1泛素连接酶复合体结合，参与错误折叠蛋白的降解。突变分析显示其锌指结构域是互作关键，提示ZER1在细胞应激反应中的调控作用。
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**备注**：ZER1的研究相对有限，上述内容为模拟简化版摘要。建议通过PubMed或Web of Science以“ZER1 recombinant”或“ZER1 protein function”为关键词获取最新文献，并注意可能存在别名(如ZYG11A)。
**Background of Recombinant Human ZER1 Protein**
Recombinant human ZER1 protein is a genetically engineered protein produced using heterologous expression systems, such as *E. coli* or mammalian cells, enabling studies of its structure and function. ZER1 (Zyg-11 related cell cycle regulator 1) is a substrate-recognition component of the Cullin-RING E3 ubiquitin ligase complex (CRL2^ZER1), which plays a critical role in the ubiquitin-proteasome pathway by targeting specific proteins for degradation.
ZER1 is implicated in regulating cellular processes, including embryonic development, cell cycle progression, and signal transduction. It forms a complex with CUL2. elongin B (ELOB), and elongin C (ELOC), recognizing substrates like anti-apoptotic proteins or transcription factors to modulate cell survival and differentiation. Dysregulation of ZER1 has been linked to diseases such as cancer, neurodegenerative disorders, and developmental abnormalities, highlighting its importance in maintaining homeostasis.
Structurally, ZER1 contains conserved domains essential for substrate binding and interactions with the CRL2 complex. Despite its known roles, the precise molecular mechanisms and full spectrum of physiological/pathological functions remain under investigation. Recombinant ZER1 serves as a vital tool for unraveling its biological significance, exploring therapeutic targets, and modeling disease mechanisms in biomedical research. Further studies aim to clarify its substrate specificity, regulatory networks, and potential clinical applications.
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