| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC7 |
| Uniprot No | Q9NXF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208 aa |
| 活性数据 | MLTDPGAVPKGNATKEYMESLQLKPGEVIYKCPKCCCIKPERAHHCSICKRCIRKMDHHCPWVNNCVGEKNQRFFVLFTMYIALSSVHALILCGFQFISCVRGQWTECSDFSPPITVILLIFLCLEGLLFFTFTAVMFGTQIHSICNDETEIERLKSEKPTWERRLRWEGMKSVFGGPPSLLWMNPFVGFRFRRLPTRPRKGGPEFSV |
| 分子量 | 48.62 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZDHHC7蛋白的3篇参考文献的概括(注：以下内容为模拟示例，具体文献需通过学术数据库验证)：
1. **《ZDHHC7-mediated palmitoylation regulates synaptic targeting of PSD-95》**
*作者：Fukata, M. et al. (2016)*
摘要：研究揭示了ZDHHC7作为棕榈酰转移酶，通过其N端结构域特异性识别并催化突触后蛋白PSD-95的棕榈酰化，调控其在神经元突触中的定位及功能，影响突触可塑性。
2. **《ZDHHC7 promotes tumor metastasis via palmitoylation-dependent EGFR signaling activation》**
*作者：Ko, P. J. & Dixon, J. E. (2018)*
摘要：该文献发现ZDHHC7在多种癌症中高表达，通过棕榈酰化修饰EGFR受体，增强下游MAPK信号通路活性，从而促进肿瘤细胞迁移和侵袭，提示其作为潜在治疗靶点。
3. **《Loss of ZDHHC7 leads to impaired AMPA receptor trafficking and cognitive deficits in Alzheimer’s models》**
*作者：Datta, D. et al. (2020)*
摘要：研究表明，ZDHHC7缺失导致AMPA受体亚基GluA1的棕榈酰化减少，加剧阿尔茨海默病模型小鼠的突触功能障碍和认知衰退，重组ZDHHC7蛋白可部分挽救这一表型。
**注意**：以上文献信息为示例，实际引用请通过PubMed或Web of Science等平台核对作者、年份及摘要准确性。建议结合关键词“ZDHHC7”、“palmitoylation”、“recombinant”进一步检索近期研究。
ZDHHC7. a member of the DHHC (Asp-His-His-Cys) domain-containing protein family, is a palmitoyltransferase enzyme involved in post-translational protein palmitoylation. This reversible lipid modification regulates protein-membrane interactions, trafficking, stability, and function. Human ZDHHC7 (zinc finger DHHC-type containing 7) is encoded by the ZDHHC7 gene located on chromosome 16q22.1 and consists of seven exons. The enzyme contains a conserved DHHC catalytic motif within its cysteine-rich domain, flanked by multiple transmembrane regions that anchor it to endoplasmic reticulum (ER) and Golgi membranes.
Functionally, ZDHHC7 mediates S-palmitoylation of diverse substrates, including neurotransmitter receptors, signaling proteins, and viral envelope proteins. It shows substrate specificity for proteins like SNAP-25. β2-adrenergic receptor, and SSTR5 somatostatin receptor. Dysregulation of ZDHHC7 has been implicated in neurological disorders, cancer progression, and immune responses. For example, abnormal palmitoylation via ZDHHC7 may contribute to synaptic dysfunction in Alzheimer's disease and Huntington's disease.
Recombinant human ZDHHC7 protein is typically expressed in mammalian or insect cell systems to preserve proper folding and membrane association. Purification often utilizes affinity tags (e.g., His-tag) combined with detergent solubilization. This recombinant tool enables biochemical characterization, substrate screening, and structural studies to unravel its enzymatic mechanisms and develop targeted modulators. Current research focuses on mapping its interactome and validating therapeutic potential in palmitoylation-related pathologies.
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