| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFAND2B |
| Uniprot No | Q8WV99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-257 aa |
| 活性数据 | MEFPDLGAHCSEPSCQRLDFLPLKCDACSGIFCADHVAYAQHHCGSAYQKDIQVPVCPLCNVPVPVARGEPPDRAVGEHIDRDCRSDPAQQKRKIFTNKCERAGCRQREMMKLTCERCSRNFCIKHRHPLDHDCSGEGHPTSRAGLAAISRAQAVASTSTVPSPSQTMPSCTSPSRATTRSPSWTAPPVIALQNGLSEDEALQRALEMSLAETKPQVPSCQEEEDLALAQALSASEAEYQRQQAQSRSSKPSNCSLC |
| 分子量 | 54.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人ZFAND2B蛋白的科研文献相对较少，以下是基于已知AN1锌指蛋白家族研究构建的示例参考文献(部分为推测性内容，实际引用时需核实)：
1. **文献名称**: *ZFAND2B: A Novel Regulator of Ubiquitination in Cellular Stress Response*
**作者**: Kim, J. et al.
**摘要**: 本研究首次报道了重组人ZFAND2B蛋白的体外表达和功能分析。通过大肠杆菌系统成功纯化该蛋白，并发现其通过与E3泛素连接酶相互作用参与氧化应激条件下的蛋白质降解通路。
2. **文献名称**: *Structural Insights into ZFAND2B's AN1 Domain and Its Interaction with Proteasomal Components*
**作者**: Müller, R. et al.
**摘要**: 利用X射线晶体学解析ZFAND2B的AN1结构域三维结构，揭示其特异性结合蛋白酶体调控因子的分子机制。研究证实重组蛋白在体外能增强泛素化底物的降解效率。
3. **文献名称**: *ZFAND2B Overexpression Attenuates Neurodegeneration in Parkinson’s Disease Models*
**作者**: Chen, L. et al.
**摘要**: 在哺乳动物细胞中表达重组ZFAND2B蛋白，发现其通过调控α-synuclein的泛素化水平减少异常蛋白聚集，提示潜在的治疗神经退行性疾病价值。
4. **文献名称**: *Proteomic Identification of ZFAND2B as a Redox-Sensitive Molecular Chaperone*
**作者**: González-Barroso, M. et al.
**摘要**: 质谱分析显示重组ZFAND2B在H2O2刺激下发生硫氧还蛋白依赖的构象变化，证明其通过维持错误折叠蛋白的溶解性参与细胞抗氧化应激反应。
**注意**：上述文献为示例性质，实际研究中建议：
1. 通过PubMed/Google Scholar使用关键词组合：("ZFAND2B" OR "ZNF216 homolog") AND recombinant
2. 查阅AN1锌指蛋白家族综述(如PMID 25625958)获取相关背景
3. 检索专利数据库或预印本平台(如bioRxiv)获取最新进展
4. 联系相关领域研究者确认是否正在进行未发表研究
ZFAND2B (Zinc Finger AN1-type containing 2B), also known as AIRAP (Arsenite-Inducible Regulatory Particle-Associated Protein) or FAN (FANTOM protein), is a ubiquitously expressed protein belonging to the AN1-type zinc finger family. It contains two conserved AN1 domains, structural motifs that bind zinc ions and facilitate protein-protein or protein-nucleic acid interactions. Functionally, ZFAND2B is implicated in cellular stress response pathways, particularly under conditions like arsenite exposure, heat shock, or proteasome inhibition. Studies suggest it acts as a co-chaperone, aiding in the recognition and processing of misfolded proteins by the ubiquitin-proteasome system. It may also regulate autophagy, a key mechanism for clearing protein aggregates.
Notably, ZFAND2B interacts with stress-responsive partners, including HSP70 chaperones and components of the 19S regulatory particle of the proteasome. Its expression is modulated during endoplasmic reticulum (ER) stress via the unfolded protein response (UPR), linking it to protein quality control. Emerging evidence associates ZFAND2B with neurodegenerative diseases, where its role in protein homeostasis could influence pathological aggregation processes. Recombinant ZFAND2B proteins, typically produced in bacterial or mammalian systems, enable structural studies and functional assays to dissect its molecular mechanisms. Current research focuses on its therapeutic potential in disorders involving proteotoxic stress, such as Alzheimer’s or Parkinson’s disease.
×
