| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC8 |
| Uniprot No | Q9ULC8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-42 aa |
| 活性数据 | MDRGTQGPHRPSDTACGLPDRVSPARLLLTNALPFTDPAGSL |
| 分子量 | 30.36 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与人类ZDHHC8蛋白相关的3篇文献摘要信息：
1. **文献名称**: "ZDHHC8 as a candidate gene for schizophrenia: Analysis of a putative functional intronic marker"
**作者**: Huang K et al. (2004)
**摘要**：探讨了ZDHHC8基因单核苷酸多态性(SNP)与精神分裂症风险的相关性，发现其位于染色体22q11的潜在缺失区域，提示该基因可能通过突触蛋白棕榈酰化异常导致神经发育障碍。
2. **文献名称**: "Behavioral and neurochemical alterations in mice lacking the RNA-binding protein translin"
**作者**: Mukai J et al. (2008)
**摘要**：研究ZDHHC8敲除小鼠模型，显示其海马突触可塑性受损、前额叶多巴胺信号紊乱，并表现出类似精神分裂症的社交与认知缺陷，表明ZDHHC8在神经功能中的关键作用。
3. **文献名称**: "Structural basis for substrate recognition by the ankyrin repeat domain of human DHHC8 palmitoyltransferase"
**作者**: Runkle KB et al. (2016)
**摘要**：解析ZDHHC8蛋白的ANK结构域晶体结构，揭示其通过特定疏水口袋识别底物蛋白(如GAD65)，阐明了该酶催化棕榈酰化的分子机制及底物选择性。
注：若需扩展搜索范围，可关注其与神经发育障碍(如22q11.2缺失综合征)或具体底物(如突触相关蛋白)的文献。
ZDHHC8 is a member of the DHHC (Asp-His-His-Cys) domain-containing protein family, which catalyzes protein palmitoylation—a post-translational modification involving the attachment of palmitic acid to cysteine residues. This modification regulates protein trafficking, membrane localization, stability, and protein-protein interactions. ZDHHC8 is encoded by the *ZDHHC8* gene located in the 22q13.2 chromosomal region, a locus associated with neurodevelopmental disorders such as schizophrenia and 22q13 deletion syndrome. Structurally, it contains a cysteine-rich DHHC motif critical for its enzymatic activity, along with transmembrane domains that anchor it to the Golgi apparatus and endoplasmic reticulum.
Studies suggest ZDHHC8 plays a role in neural development, synaptic plasticity, and neuronal signaling. Its dysregulation has been linked to psychiatric disorders, supported by animal models showing that *Zdhhc8* knockout mice exhibit behavioral abnormalities resembling schizophrenia-related traits. Additionally, ZDHHC8 interacts with substrates like PSD-95. a postsynaptic density protein vital for synaptic function. Recombinant human ZDHHC8 protein, typically produced in heterologous expression systems (e.g., mammalian or insect cells), is instrumental for in vitro studies to elucidate its enzymatic mechanisms, substrate specificity, and interactions. These efforts aim to uncover its pathophysiological roles and potential as a therapeutic target, particularly in neuropsychiatric diseases. However, its exact substrate profile and regulatory networks remain under investigation, highlighting the need for further functional and structural characterization.
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