关键信息
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基因名
MAPKAPK3
- 应用
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别名
MAPKAPK3；MAP kinase-activated Protein kinase 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q16644
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表达区间
1-382aa
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氨基酸序列
MDGETAEEQG GPVPPPVAPG GPGLGGAPGG RREPKKYAVT DDYQLSKQVL GLGVNGKVLE CFHRRTGQKC ALKLLYDSPK ARQEVDHHWQ ASGGPHIVCI LDVYENMHHG KRCLLIIMEC MEGGELFSRI QERGDQAFTE REAAEIMRDI GTAIQFLHSH NIAHRDVKPE NLLYTSKEKD AVLKLTDFGF AKETTQNALQ TPCYTPYYVA PEVLGPEKYD KSCDMWSLGV IMYILLCGFP PFYSNTGQAI SPGMKRRIRL GQYGFPNPEW SEVSEDAKQL IRLLLKTDPT ERLTITQFMN HPWINQSMVV PQTPLHTARV LQEDKDHWDE VKEEMTSALA TMRVDYDQVK IKDLKTSNNR LLNKRRKKQA GSSSASQGCN NQ
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MAPKAPK3, or Mitogen-Activated Protein Kinase-Activated Protein Kinase 3, is a member of the MAPK-activated protein kinase family, which plays a pivotal role in cellular signaling pathways, particularly those related to stress responses, inflammation, and cell proliferation. Understanding the function and regulation of MAPKAPK3 is crucial, as it is implicated in various pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders. Research has shown that MAPKAPK3 acts downstream of the MAPK signaling cascade and is activated by various stimuli, including growth factors and stress signals. This protein influences several cellular processes by phosphorylating target substrates involved in transcriptional regulation, cell cycle control, and apoptosis. The recombinant expression of MAPKAPK3 in various systems facilitates the study of its functional properties and interactions with other proteins, enabling the exploration of its potential as a therapeutic target. Additionally, investigations into the structural characteristics of MAPKAPK3 through crystallography and other methods shed light on its mechanism of action and regulation. Overall, the study of MAPKAPK3 is significant for elucidating its role in health and disease, paving the way for the development of novel therapeutic strategies aimed at modulating its activity.
