| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC4 |
| Uniprot No | Q9NPG8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344 aa |
| 活性数据 | MDFLVLFLFYLASVLMGLVLICVCSKTHSLKGLARGGAQIFSCIIPECLQRAVHGLLHYLFHTRNHTFIVLHLVLQGMVYTEYTWEVFGYCQELELSLHYLLLPYLLLGVNLFFFTLTCGTNPGIITKANELLFLHVYEFDEVMFPKNVRCSTCDLRKPARSKHCSVCNWCVHRFDHHCVWVNNCIGAWNIRYFLIYVLTLTASAATVAIVSTTFLVHLVVMSDLYQETYIDDLGHLHVMDTVFLIQYLFLTFPRIVFMLGFVVVLSFLLGGYLLFVLYLAATNQTTNEWYRGDWAWCQRCPLVAWPPSAEPQVHRNIHSHGLRSNLQEIFLPAFPCHERKKQE |
| 分子量 | 66.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人源ZDHHC4蛋白的3篇代表性文献的简要信息(基于当前知识库内容生成，部分文献为模拟举例)：
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1. **文献名称**: *ZDHHC4-mediated palmitoylation of PTEN modulates glioma progression*
**作者**: Li et al. (2020)
**摘要**: 研究发现ZDHHC4通过催化肿瘤抑制因子PTEN的棕榈酰化修饰，促进其膜定位和稳定性，从而抑制胶质母细胞瘤的增殖和侵袭。重组人ZDHHC4蛋白的体外实验证实其酶活性对PTEN功能调控至关重要。
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2. **文献名称**: *Structural basis of substrate recognition by human ZDHHC4 palmitoyltransferase*
**作者**: Wang et al. (2021)
**摘要**: 本研究解析了重组人ZDHHC4蛋白的晶体结构，揭示了其底物结合域的独特构象，并通过突变分析鉴定了催化棕榈酰化反应的关键氨基酸残基，为设计靶向ZDHHC4的小分子抑制剂奠定基础。
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3. **文献名称**: *ZDHHC4 deficiency promotes hepatocellular carcinoma metastasis via lipid metabolic reprogramming*
**作者**: Chen et al. (2022)
**摘要**: 利用重组ZDHHC4蛋白及敲除模型，证明ZDHHC4通过棕榈酰化修饰脂肪酸代谢酶ACSL4.调控肝癌细胞脂代谢并抑制EMT过程。临床数据分析显示ZDHHC4低表达与肝癌患者预后不良相关。
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*注：以上为示例性文献，实际引用请以真实发表的论文为准。建议通过PubMed或Web of Science搜索最新研究。*
The ZDHHC4 protein, a member of the DHHC (Asp-His-His-Cys) palmitoyltransferase family, plays a critical role in protein palmitoylation—a post-translational modification essential for membrane trafficking, protein stability, and cellular signaling. Located on human chromosome 7. ZDHHC4 contains a conserved DHHC domain flanked by cysteine-rich regions and transmembrane segments, enabling its enzymatic activity and membrane association. It catalyzes the addition of palmitate (a fatty acid) to specific cysteine residues of target proteins, modulating their localization and function.
Though less characterized than other DHHC members, ZDHHC4 is implicated in neurodegenerative diseases and cancer. Studies suggest its involvement in regulating autophagy and apoptosis, potentially through interactions with proteins like BCL2 and LC3. Dysregulation of ZDHHC4 has been linked to hepatocellular carcinoma (HCC), where overexpression correlates with poor prognosis.
Recombinant human ZDHHC4 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and is widely used to study palmitoylation mechanisms *in vitro*. Its biochemical properties, substrate specificity, and structural insights are critical for understanding disease pathways and developing targeted therapies. Current research focuses on identifying its physiological substrates and exploring its therapeutic potential in oncology and neurodegeneration.
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