| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC6 |
| Uniprot No | Q9H6R6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-409 aa |
| 活性数据 | MGTFCSVIKFENLQELKRLCHWGPIIALGVIAICSTMAMIDSVLWYWPLHTTGGSVNFIMLINWTVMILYNYFNAMFVGPGFVPLGWKPDTMYLQYCKVCQAYKAPRSHHCRKCNRCVMKMDHHCPWINNCCGYQNHASFTLFLLLAPLGCIHAAFIFVMTMYTQLYHRLSFGWNTVKIDMSAARRDPLPIVPFGLAAFATTLFALGLALGTTIAVGMLFFIQMKIILRNKTSIESWIEEKAKDRIQYYQLDEVFVFPYDMGSRWRNFKQVFTWSGVPEGDGLEWPVREGCHQYSLTIEQLKQKADKRVRSVRYKVIEDYSGACCPLNKGIKTFFTSPCTEEPRIQLQKGEFILATRGLRYWLYGDKILDDSFIEGVSRIRGWFPRKCVEKCPCDAETDQAPEGEKKNR |
| 分子量 | 70.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZDHHC6蛋白的3篇代表性文献及其摘要内容的示例(文献标题和作者为示例性描述，建议通过PubMed或学术数据库获取真实原文信息)：
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1. **文献名称**：*ZDHHC6 regulates B cell receptor signaling through palmitoylation of CD79A*
**作者**：Smith A et al.
**摘要**：该研究发现ZDHHC6作为棕榈酰转移酶，特异性催化B细胞受体(BCR)信号分子CD79A的棕榈酰化修饰，影响其膜定位及下游信号传导，揭示了ZDHHC6在B细胞免疫应答中的关键作用。
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2. **文献名称**：*The ER-localized enzyme ZDHHC6 modulates the unfolded protein response by palmitoylating IRE1α*
**作者**：Chen L et al.
**摘要**：研究证实ZDHHC6通过棕榈酰化修饰内质网应激传感器IRE1α，增强其激酶活性并促进未折叠蛋白反应(UPR)信号通路，提示ZDHHC6在维持内质网稳态中的生理功能。
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3. **文献名称**：*Structural insights into the catalytic mechanism of ZDHHC6 in protein palmitoylation*
**作者**：Wang Y et al.
**摘要**：通过冷冻电镜解析了ZDHHC6的晶体结构，阐明了其催化底物棕榈酰化的分子机制，并鉴定了关键活性位点，为靶向ZDHHC6的药物开发提供理论基础。
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**注意事项**：
以上文献信息为简化示例，实际研究中需通过**PubMed**、**Web of Science**或期刊官网检索真实文献(关键词：ZDHHC6. palmitoyltransferase, palmitoylation)。该蛋白的研究多聚焦于其酶活性调控、底物特异性及在疾病(如癌症、神经退行性疾病)中的作用。
ZDHHC6 (Zinc Finger DHHC-Type Containing 6) is a member of the DHHC-family of palmitoyltransferases, enzymes responsible for post-translational S-palmitoylation—a reversible lipid modification critical for protein trafficking, stability, and function. This 34-kDa endoplasmic reticulum (ER)-localized protein contains a conserved DHHC (Asp-His-His-Cys) catalytic motif and four transmembrane domains. It specifically palmitoylates substrates like B-cell receptor signaling components and ER-resident proteins (e.g., calnexin), influencing ER homeostasis, protein folding, and stress responses. Studies link ZDHHC6 dysfunction to diseases, including cancer (breast, colon) and neurodegenerative disorders, due to disrupted palmitoylation-dependent pathways. Recombinant human ZDHHC6 protein, often expressed in mammalian or insect cell systems, enables in vitro studies of its enzymatic mechanisms, substrate specificity, and inhibitor screening. Recent research focuses on its regulatory interplay with ER stressors like ATF6 and its potential as a therapeutic target, given its role in modulating cell survival under stress. Structural analysis of recombinant ZDHHC6 reveals conformational dynamics essential for substrate recognition, offering insights into enzyme engineering or drug design.
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