| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC24 |
| Uniprot No | Q6UX98 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-284 aa |
| 活性数据 | MGQPWAAGSTDGAPAQLPLVLTALWAAAVGLELAYVLVLGPGPPPLGPLARALQLALAAFQLLNLLGNVGLFLRSDPSIRGVMLAGRGLGQGWAYCYQCQSQVPPRSGHCSACRVCILRRDHHCRLLGRCVGFGNYRPFLCLLLHAAGVLLHVSVLLGPALSALLRAHTPLHMAALLLLPWLMLLTGRVSLAQFALAFVTDTCVAGALLCGAGLLFHGMLLLRGQTTWEWARGQHSYDLGPCHNLQAALGPRWALVWLWPFLASPLPGDGITFQTTADVGHTAS |
| 分子量 | 56.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZDHHC24蛋白的**模拟参考文献示例**(请注意，这些文献为虚构示例，实际研究需通过学术数据库验证)：
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1. **"Functional analysis of recombinant human ZDHHC24 palmitoyltransferase in lipid metabolism"**
*作者：Chen et al.*
**摘要**：研究重组表达的ZDHHC24蛋白的酶学特性，证实其在体外催化特定膜蛋白的棕榈酰化修饰，并揭示其在脂质代谢通路中的潜在调控作用。
2. **"Crystal structure of the catalytic domain of ZDHHC24 reveals insights into substrate recognition"**
*作者：Johnson & Müller*
**摘要**：通过重组蛋白技术获得ZDHHC24的催化结构域，解析其三维结构，阐明其与底物结合的分子机制及保守性DHHC基序的功能。
3. **"ZDHHC24-mediated palmitoylation promotes epidermal barrier formation in human keratinocytes"**
*作者：Tanaka et al.*
**摘要**：利用重组ZDHHC24蛋白进行体外实验，发现其通过棕榈酰化角蛋白相关蛋白，参与皮肤屏障功能的维持，为皮肤病机制提供新视角。
4. **"Role of ZDHHC24 in neurodegenerative disease: Evidence from recombinant protein interaction studies"**
*作者：Wang et al.*
**摘要**：重组ZDHHC24与淀粉样前体蛋白(APP)的相互作用实验表明，其可能通过调控APP棕榈酰化影响阿尔茨海默病相关通路。
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**提示**：实际研究中，建议通过PubMed、Google Scholar等平台以关键词“ZDHHC24 recombinant”、“ZDHHC24 palmitoyltransferase”检索最新文献，或关注该蛋白在特定疾病(如癌症、神经退行性疾病)中的功能研究。
ZDHHC24 is a member of the zinc finger DHHC-type (ZDHHC) protein family, which comprises palmitoyltransferases involved in post-translational protein S-palmitoylation. This enzymatic process regulates protein trafficking, membrane localization, and stability. Human ZDHHC24. encoded by the ZDHHC24 gene, contains a conserved DHHC catalytic domain flanked by transmembrane regions and cytoplasmic termini. It is predominantly expressed in epidermal keratinocytes and plays a role in skin barrier formation by palmitoylating structural proteins critical for cornified envelope assembly. Dysregulation of ZDHHC24 has been linked to dermatological disorders, including psoriasis, where its overexpression may disrupt epidermal differentiation. Additionally, emerging studies suggest potential involvement in cancer progression through aberrant palmitoylation of oncogenic substrates. Recombinant human ZDHHC24 protein is typically produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its recombinant form enables in vitro analysis of substrate specificity, enzymatic kinetics, and interaction networks. Current research focuses on characterizing its structural domains, identifying physiological substrates, and exploring therapeutic targeting of its activity for skin diseases or malignancies. Challenges remain in elucidating its substrate recognition mechanisms and regulatory pathways due to the complexity of palmitoylation dynamics in cellular contexts.
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