| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC21 |
| Uniprot No | Q8IVQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-265 aa |
| 活性数据 | MGLRIHFVVDPHGWCCMGLIVFVWLYNIVLIPKIVLFPHYEEGHIPGILIIIFYGISIFCLVALVRASITDPGRLPENPKIPHGEREFWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCFYTELLTCYALMFSFCHYYYFLPLKKRNLDLFVFRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTTSIEKMSNCCEDISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRVPYHFANHV |
| 分子量 | 57.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人ZDHHC21蛋白的文献摘要示例：
1. **文献名称**: *ZDHHC21 regulates keratinocyte differentiation via palmitoylation of ΔNp63α*
**作者**: Li et al., 2021
**摘要**: 研究揭示了ZDHHC21通过棕榈酰化修饰转录因子ΔNp63α，调控表皮角质形成细胞分化和皮肤屏障功能。实验表明，重组人ZDHHC21蛋白具有特异性棕榈酰转移酶活性，且其功能缺失导致小鼠皮肤发育异常。
2. **文献名称**: *Structural insights into ZDHHC21-mediated protein S-palmitoylation*
**作者**: Chen & Wang, 2019
**摘要**: 通过冷冻电镜解析了人源ZDHHC21蛋白的催化结构域结构，发现其底物结合口袋的独特构象。重组ZDHHC21在体外成功催化了底物蛋白的棕榈酰化修饰，并提出其活性受膜结合结构域调控的机制。
3. **文献名称**: *ZDHHC21 promotes breast cancer metastasis by palmitoylating SNAP-23*
**作者**: Zhang et al., 2020
**摘要**: 报道ZDHHC21在乳腺癌中高表达，通过棕榈酰化SNARE蛋白SNAP-23促进肿瘤细胞侵袭和转移。利用重组ZDHHC21蛋白验证了其与SNAP-23的相互作用及酶活依赖性功能，提示其作为治疗靶点的潜力。
*注：以上文献为示例，实际引用需根据具体研究内容选择近年高质量论文(如Nature、Cell或JBC等期刊)。建议通过PubMed或Google Scholar以“ZDHHC21 recombinant protein”“ZDHHC21 palmitoyltransferase”等关键词检索最新文献。*
The ZDHHC21 protein, a member of the ZDHHC (zinc finger DHHC-type containing) family, is a palmitoyltransferase that catalyzes the post-translational addition of palmitate to specific cysteine residues of target proteins. This lipid modification, known as S-palmitoylation, regulates protein trafficking, stability, membrane localization, and interactions. ZDHHC21 contains a conserved DHHC motif within its catalytic domain and multiple transmembrane regions, enabling its localization to the Golgi apparatus or endoplasmic reticulum. It exhibits tissue-specific expression, with high levels in the brain, testis, and ovaries, suggesting roles in neuronal function and reproductive biology.
Emerging studies link ZDHHC21 to diverse physiological and pathological processes. It palmitoylates substrates involved in neurotransmission (e.g., SNAP-25), immune signaling, and cancer progression (e.g., Wnt proteins). Dysregulation of ZDHHC21 is implicated in neurodegenerative disorders, metabolic syndromes, and malignancies. For instance, loss-of-function mutations correlate with intellectual disability, while overexpression in cancers may drive proliferation. Recombinant ZDHHC21 protein, typically produced via mammalian or insect cell systems, is utilized to study enzymatic mechanisms, substrate specificity, and inhibitor screening. Its structural complexity and membrane-associated nature pose challenges for purification, necessitating detergent-based solubilization strategies. Current research focuses on elucidating its substrate networks and therapeutic potential in diseases linked to palmitoylation defects.
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