关键信息
-
基因名
Cyp3a13
- 应用
-
别名
Cyp3a13；Cyp3a-13；Cytochrome P450 3A13
-
种属
Mouse
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q64464
-
表达区间
1-503aa
-
氨基酸序列
MDLIPNFSMETWMLLATSLVLLYLYGTHSHGIFKKLGIPGPKPLPFLGTILAYQKGFWECDIQCHKKYGKMWGLYDGRQPVLAITDPDIIKTVLVKECYSTFTNRRRFGPVGILKKAISISENEEWKRIRALLSPTFTSGRLKEMFPIINQFTDVLVRNMRQGLGEGKPTSMKDIFGAYSMDVITATSFGVNIDSLNNPQDPFVEKIKKLLKFDIFDPLFLSVTLFPFLTPVFDALNVSLFPRDVISFFTTSVERMKENRMKEKEKQRVDFLQLMINSQNYKTKESHKALSDVEIVAQSVIFIFAGYETTSSALSFALYLLAIHPDVQKKLQDEIDAALPNKAPATYDTLLQMEYLDMVVNETLRLYPIAGRLERVCKTDVEINGLFIPKGTVVMIPTFALHKDPKYWPEPEEFRPERFSKKNQDSINPYMYLPFGSGPRNCIGMRFALINMKVALVRVLQNFTVQPCKETEIPLKLSKQGLLQPENPLLLKVVSRDETVSDE
-
分子量
73.5 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CYP3A13 is a member of the cytochrome P450 family of enzymes, which play a crucial role in the metabolism of various xenobiotics and endogenous compounds. Particularly, CYP3A13 is predominantly expressed in the liver and is involved in the metabolism of many drugs, including statins, antidepressants, and antiretrovirals. Its importance in pharmacogenomics cannot be overstated, as genetic polymorphisms in CYP3A13 can influence individual responses to medication, leading to variations in drug efficacy and the risk of adverse effects. The enzyme's role in drug metabolism and detoxification is vital for understanding inter-individual variability in drug clearance and toxicity. Research into CYP3A13 recombinant protein aims to elucidate its enzymatic mechanisms, substrate specificity, and regulation, providing insights into how genetic variations can impact enzyme function. These studies hold significant implications in clinical settings, particularly in personalized medicine, where understanding an individual's metabolic capabilities can lead to more effective and safer therapeutic strategies. Furthermore, investigations into CYP3A13 could enhance our understanding of drug-drug interactions and the potential impact of environmental factors on enzyme activity. As such, recombinant CYP3A13 serves as a valuable tool in both basic research and applied sciences, contributing to the development of better therapeutic regimens and promoting safe drug usage across diverse populations.
