| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC1 |
| Uniprot No | Q8WTX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-485 aa |
| 活性数据 | MYKMNICNKPSNKTAPEKSVWTAPAQPSGPSPELQGQRSRRNGWSWPPHPLQIVAWLLYLFFAVIGFGILVPLLPHHWVPAGYACMGAIFAGHLVVHLTAVSIDPADANVRDKSYAGPLPIFNRSQHAHVIEDLHCNLCNVDVSARSKHCSACNKCVCGFDHHCKWLNNCVGERNYRLFLHSVASALLGVLLLVLVATYVFVEFFVNPMRLRTNRHFEVLKNHTDVWFVFLPAAPVETQAPAILALAALLILLGLLSTALLGHLLCFHIYLMWHKLTTYEYIVQHRPPQEAKGVHRELESCPPKMRPIQEMEFYMRTFRHMRPEPPGQAGPAAVNAKHSRPASPDPTPGRRDCAGPPVQVEWDRKKPLPWRSPLLLLAMWGPQAPPCLCRKRGRGACIKCERLRPRIRRRGLGPPAAAPARRRIPRTPALCTPLALPAPTTRRRQSPWTRFQWRRRAWAAPLWPPRGAGADSPRWRGRRVRPPFS |
| 分子量 | 81.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZDHHC1蛋白的3篇参考文献(模拟示例，基于领域内常见研究方向构建)：
1. **文献名称**：*Structural and functional analysis of human ZDHHC1 palmitoyltransferase*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌表达重组人ZDHHC1蛋白，解析了其晶体结构，证明其依赖锌离子的催化活性，并鉴定出底物结合的关键氨基酸位点。
2. **文献名称**：*ZDHHC1-mediated palmitoylation regulates synaptic protein trafficking in neurons*
**作者**：Chen B, et al.
**摘要**：利用重组ZDHHC1蛋白进行体外棕榈酰化实验，发现其特异性修饰突触蛋白PSD-95.调控其在神经元中的定位及突触可塑性。
3. **文献名称**：*Dysregulation of ZDHHC1 in cancer: Role in EGFR signaling*
**作者**：Wang L, et al.
**摘要**：通过哺乳动物细胞表达重组ZDHHC1.揭示其在乳腺癌细胞中通过棕榈酰化修饰EGFR，增强下游信号通路并促进肿瘤侵袭。
*注：以上文献为模拟示例，实际研究需根据具体数据库检索。如需真实文献，建议通过PubMed或Web of Science搜索关键词“ZDHHC1 recombinant”“ZDHHC1 palmitoylation”。*
ZDHHC1 (zinc finger DHHC-type containing 1) is a member of the DHHC protein family, characterized by a conserved catalytic domain (DHHC-CRD) that enables palmitoyltransferase activity. This enzyme facilitates protein S-palmitoylation, a post-translational modification where palmitate is attached to cysteine residues, influencing substrate localization, stability, and interactions. Predominantly localized in the Golgi apparatus and endoplasmic reticulum, ZDHHC1 regulates diverse cellular processes, including intracellular trafficking, signal transduction, and membrane organization.
Studies suggest its involvement in neuronal function, immune regulation, and cancer progression. For instance, ZDHHC1-mediated palmitoylation modulates neurotransmitter receptors and adhesion molecules, impacting synaptic plasticity. Dysregulation of ZDHHC1 has been linked to pathological conditions, such as neurodegenerative disorders and tumor metastasis, though its specific mechanisms remain under investigation.
Recombinant human ZDHHC1 protein, produced via bacterial or mammalian expression systems, is widely used to study enzymatic kinetics, substrate identification, and interaction networks. Its application in vitro and in vivo models aids in deciphering palmitoylation’s role in health and disease. Despite progress, comprehensive understanding of ZDHHC1’s substrate specificity and regulatory pathways requires further exploration, highlighting its potential as a therapeutic target.
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