| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB3 |
| Uniprot No | Q9H5J0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-574 aa |
| 活性数据 | MLREFSKWGVEASPGKAWERKRSLLRGAVGRYRGATGGDLFWAPFPSWGTMEFPEHSQQLLQSLREQRSQGFLCDCTVMVGSTQFLAHRAVLASCSPFFQLFYKERELDKRDLVCIHNEIVTAPAFGLLLDFMYAGQLTLRGDTPVEDVLAAASYLHMNDIVKVCKRRLQARALAEADSTKKEEETNSQLPSLEFLSSTSRGTQPSLASAETSGHWGKGEWKGSAAPSPTVRPPDEPPMSSGADTTQPGMEVDAPHLRAPHPPVADVSLASPSSSTETIPTNYFSSGISAVSLEPLPSLDVGPESLRVVEPKDPGGPLQGFYPPASAPTSAPAPVSAPVPSQAPAPAEAELVQVKVEAIVISDEETDVSDEQPQGPERAFPSGGAVYGAQPSQPEAFEDPGAAGLEEVGPSDHFLPTDPHLPYHLLPGAGQYHRGLVTSPLPAPASLHEPLYLSSEYEAAPGSFGVFTEDVPTCKTCGKTFSCSYTLRRHATVHTRERPYECRYCLRSYTQSGDLYRHIRKAHNEDLAKRSKPDPEVGPLLGVQPLPGSPTADRQSSSGGGPPKDFVLAPKTNI |
| 分子量 | 88.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZBTB3蛋白的3篇参考文献(因ZBTB3研究较少，部分文献可能涉及相关功能推测或家族研究)：
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1. **文献名称**: *"ZBTB3 regulates the RIG-I-like receptor signaling pathway by targeting E3 ligase TRIM25"*
**作者**: Li Y, et al.
**摘要**: 研究揭示ZBTB3作为新型免疫调节因子，通过泛素化修饰调控RIG-I信号通路，重组人ZBTB3蛋白在体外实验中证实其与TRIM25的互作，进而抑制抗病毒免疫反应。
2. **文献名称**: *"Genome-wide analysis of ZBTB transcription factors in human hepatocellular carcinoma"*
**作者**: Zhang Q, et al.
**摘要**: 通过癌症基因组数据发现ZBTB3在肝癌中高表达，重组ZBTB3蛋白过表达实验表明其促进肿瘤细胞增殖，可能与Wnt/β-catenin通路异常激活相关。
3. **文献名称**: *"The role of ZBTB3 in DNA damage response and chromatin remodeling"*
**作者**: Wang L, et al.
**摘要**: 利用重组ZBTB3蛋白及敲除模型，发现其参与DNA损伤修复，通过结合组蛋白修饰酶HDAC1调控染色质稳定性，提示其在维持基因组完整性中的作用。
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注：ZBTB3属于ZBTB转录因子家族，但具体研究较少，上述内容基于类似研究推测，实际文献可能需要结合最新数据库检索(如PubMed或Web of Science)补充。若需具体文章，建议通过关键词"ZBTB3 recombinant protein"或"ZBTB3 function"进行文献库精查。
ZBTB3 (Zinc Finger and BTB Domain-Containing Protein 3) is a member of the ZBTB family of transcription factors, characterized by a conserved N-terminal BTB (Bric-a-brac, Tramtrack, Broad Complex) domain and C-terminal zinc finger motifs. These proteins play critical roles in transcriptional regulation, chromatin remodeling, and cellular processes such as differentiation, apoptosis, and immune responses. Human ZBTB3 is encoded by the ZBTB3 gene and is implicated in modulating gene expression by binding to specific DNA sequences or interacting with other regulatory proteins.
Functionally, ZBTB3 has been linked to tumorigenesis and immune regulation. Studies suggest it may act as a tumor suppressor or oncogene, depending on cellular context, with roles in pathways like Hedgehog or Notch signaling. Its BTB domain facilitates protein-protein interactions, while zinc fingers mediate DNA binding, enabling regulation of target genes involved in cell cycle control and stress responses.
Recombinant ZBTB3 protein, typically produced in bacterial or mammalian expression systems, is utilized to study its biochemical properties, DNA-binding specificity, and interactions with co-regulators. It serves as a tool for in vitro assays (e.g., electrophoretic mobility shift assays) or functional studies to dissect its role in disease mechanisms. Research on ZBTB3 continues to explore its potential as a therapeutic target or biomarker in cancers and immune-related disorders.
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