| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB37 |
| Uniprot No | Q5TC79 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-361 aa |
| 活性数据 | MEKGGNIQLEIPDFSNSVLSHLNQLRMQGRLCDIVVNVQGQAFRAHKVVLAASSPYFRDHMSLNEMSTVSISVIKNPTVFEQLLSFCYTGRICLQLADIISYLTAASFLQMQHIIDKCTQILEGIHFKINVAEVEAELSQTRTKHQERPPESHRVTPNLNRSLSPRHNTPKGNRRGQVSAVLDIRELSPPEESTSPQIIEPSSDVESREPILRINRAGQWYVETGVADRGGRSDDEVRVLGAVHIKTENLEEWLGPENQPSGEDGSSAEEVTAMVIDTTGHGSVGQENYTLGSSGAKVARPTSSEVDRFSPSGSVVPLTERHRARSESPGRMDEPKQPSSQVWSCGFRTALVVGGIATVYE |
| 分子量 | 66.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZBTB37蛋白的3篇文献示例(内容为虚构，供参考，实际文献需检索数据库确认)：
1. **"Structural Insights into Human ZBTB37 by Recombinant Expression and Crystallography"**
*作者：Zhang Y. et al.*
**摘要**：通过重组表达纯化人ZBTB37蛋白，解析其晶体结构，揭示其锌指结构域与DNA结合的分子机制。
2. **"ZBTB37 Functions as a Transcriptional Repressor in Hematopoietic Development"**
*作者：Li X. & Wang H.*
**摘要**：利用重组ZBTB37蛋白研究其在造血干细胞分化中的功能，证明其通过抑制特定靶基因调控细胞命运。
3. **"ZBTB37 Interacts with HDACs to Modulate Chromatin Remodeling"**
*作者：Chen L. et al.*
**摘要**：通过体外重组蛋白结合实验，揭示ZBTB37与组蛋白去乙酰化酶(HDACs)相互作用，参与表观遗传调控。
4. **"Aberrant ZBTB37 Expression in Colorectal Cancer and Its Oncogenic Role"**
*作者：Kim S. et al.*
**摘要**：研究重组ZBTB37在结直肠癌细胞中的异常表达，发现其通过调控Wnt/β-catenin通路促进肿瘤进展。
(注：以上文献为模拟示例，实际需通过PubMed、Google Scholar等平台以“ZBTB37”或“recombinant ZBTB37”为关键词检索真实文献。)
ZBTB37. also known as zinc finger and BTB domain-containing protein 37. is a transcription regulatory protein belonging to the ZBTB family, characterized by zinc finger motifs and a BTB (Bric-à-brac, Tramtrack, Broad Complex) domain. It functions as a nuclear transcriptional repressor, primarily involved in modulating gene expression by binding to specific DNA sequences or interacting with chromatin-remodeling complexes. The protein is conserved across vertebrates and plays critical roles in cellular differentiation, apoptosis, and cell cycle regulation.
Studies suggest its involvement in hematopoiesis and tumorigenesis, with evidence linking altered ZBTB37 expression to hematopoietic malignancies and solid tumors. For instance, it may act as a tumor suppressor by repressing oncogenic pathways like MYC signaling. Structurally, recombinant human ZBTB37 is typically expressed in bacterial or mammalian systems for experimental studies, allowing investigation of its post-translational modifications, such as phosphorylation, which influence its stability and activity.
Research remains ongoing to elucidate its precise molecular mechanisms, including interactions with co-repressors and target gene networks. Its dual zinc finger and BTB domains enable both DNA binding and protein-protein interactions, making it a key candidate for studying epigenetic regulation. While functional data are still emerging, ZBTB37 is increasingly recognized as a potential biomarker or therapeutic target in cancers and developmental disorders.
×
