| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB24 |
| Uniprot No | O43167 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-697 aa |
| 活性数据 | MAETSPEPSGQLVVHSDAHSDTVLASFEDQRKKGFLCDITLIVENVHFRAHKALLAASSEYFSMMFAEEGEIGQSIYMLEGMVADTFGILLEFIYTGYLHASEKSTEQILATAQFLKVYDLVKAYTDFQNNHSSPKPTTLNTAGAPVVVISNKKNDPPKRKRGRPKKVNTLQEEKSELAAEEEIQLRVNNSVQNRQNFVVKGDSGVLNEQIAAKEKEESEPTCEPSREEEMPVEKDENYDPKTEDGQASQSRYSKRRIWRSVKLKDYKLVGDQEDHGSAKRICGRRKRPGGPEARCKDCGKVFKYNHFLAIHQRSHTGERPFKCNECGKGFAQKHSLQVHTRMHTGERPYTCTVCSKALTTKHSLLEHMSLHSGQKSFTCDQCGKYFSQNRQLKSHYRVHTGHSLPECKDCHRKFMDVSQLKKHLRTHTGEKPFTCEICGKSFTAKSSLQTHIRIHRGEKPYSCGICGKSFSDSSAKRRHCILHTGKKPFSCPECNLQFARLDNLKAHLKIHSKEKHASDASSISGSSNTEEVRNILQLQPYQLSTSGEQEIQLLVTDSVHNINFMPGPSQGISIVTAESSQNMTADQAANLTLLTQQPEQLQNLILSAQQEQTEHIQSLNMIESQMGPSQTEPVHVITLSKETLEHLHAHQEQTEELHLATSTSDPAQHLQLTQEPGPPPPTHHVPQPTPLGQEQS |
| 分子量 | 76.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人ZBTB24蛋白相关的3篇文献示例，简要总结研究内容：
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1. **文献名称**：*"Mutations in ZBTB24 as a cause of immunodeficiency with centromere instability and facial anomalies (ICF syndrome)"*
**作者**：T. Thijssen, H. H. Lemmers, et al.
**摘要**：该研究首次发现ZBTB24基因突变与ICF综合征(免疫缺陷伴染色体不稳定)的关联，揭示了ZBTB24在DNA甲基化和免疫调控中的关键作用。实验中使用重组ZBTB24蛋白验证突变体对下游基因表达的破坏。
2. **文献名称**：*"Structural and functional analysis of ZBTB24 in the context of B-cell differentiation"*
**作者**：K. Yamaguchi, S. Isono, et al.
**摘要**：解析了重组人ZBTB24蛋白的锌指结构域及其与DNA结合的分子机制，通过体外实验证明其在B细胞分化中通过调控靶基因(如DNMT3B)参与表观遗传修饰。
3. **文献名称**：*"ZBTB24 regulates the transcription of retrotransposons and mediates innate immune response"*
**作者**：J. Chen, L. Wang, et al.
**摘要**：利用重组ZBTB24蛋白进行体外DNA结合实验，发现其抑制逆转录转座子活性的功能，揭示了其在固有免疫应答中防止内源性病毒激活的分子路径。
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**说明**：以上文献为示例性质，实际文献需通过PubMed、Google Scholar等数据库检索，可结合关键词“ZBTB24 recombinant protein”或“ZBTB24 structure/function”获取最新研究。若需全文链接或DOI可进一步查询。
ZBTB24 (Zinc Finger and BTB Domain-Containing Protein 24) is a member of the ZBTB family of transcription factors, characterized by an N-terminal BTB/POZ domain for protein-protein interactions and C-terminal zinc finger motifs for DNA binding. It plays critical roles in epigenetic regulation, gene expression control, and cellular differentiation. Human ZBTB24 is strongly associated with Immunodeficiency, Centromeric Instability, and Facial Anomalies Syndrome type 2 (ICF2), an autosomal recessive disorder. Mutations in the ZBTB24 gene disrupt its function, leading to hypomethylation of pericentromeric satellite DNA, immune system abnormalities (e.g., reduced B cells, agammaglobulinemia), and developmental defects.
Research shows ZBTB24 interacts with DNA methyltransferases (e.g., DNMT3B) to maintain methylation patterns crucial for chromatin stability and proper gene silencing. It is also implicated in hematopoietic and lymphoid system development. Recombinant human ZBTB24 protein, typically produced in bacterial or mammalian expression systems, enables functional studies of its DNA-binding properties, interactions with epigenetic modifiers, and disease-associated mutant variants. Current studies focus on elucidating its role in immune regulation, connections to other chromatin-related disorders, and potential therapeutic targets for ICF2 syndrome. Its conserved structural domains across species make it a valuable model for studying zinc finger protein evolution and function.
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