| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB34 |
| Uniprot No | Q8NCN2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-500 aa |
| 活性数据 | MDSSSFIQFDVPEYSSTVLSQLNELRLQGKLCDIIVHIQGQPFRAHKAVLAASSPYFRDHSALSTMSGLSISVIKNPNVFEQLLSFCYTGRMSLQLKDVVSFLTAASFLQMQCVIDKCTQILESIHSKISVGDVDSVTVGAEENPESRNGVKDSSFFANPVEISPPYCSQGRQPTASSDLRMETTPSKALRSRLQEEGHSDRGSSGSVSEYEIQIEGDHEQGDLLVRESQITEVKVKMEKSDRPSCSDSSSLGDDGYHTEMVDGEQVVAVNVGSYGSVLQHAYSYSQAASQPTNVSEAFGSLSNSSPSRSMLSCFRGGRARQKRALSVHLHSDLQGLVQGSDSEAMMNNPGYESSPRERSARGHWYPYNERLICIYCGKSFNQKGSLDRHMRLHMGITPFVCKFCGKKYTRKDQLEYHIRGHTDDKPFRCEICGKCFPFQGTLNQHLRKNHPGVAEVRSRIESPERTDVYVEQKLENDASASEMGLDSRMEIHTVSDAPD |
| 分子量 | 55 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZBTB34蛋白的3篇文献示例(注：具体文献可能需要根据实际数据库检索调整，以下为模拟内容供参考)：
1. **文献名称**：*ZBTB34 interacts with DAXX and functions as a transcriptional repressor*
**作者**：Li X et al.
**摘要**：研究揭示了ZBTB34与DAXX蛋白的相互作用，通过重组蛋白表达验证其转录抑制功能，表明其在染色质重塑中的潜在调控作用。
2. **文献名称**：*Characterization of ZBTB34 as a novel binding partner of DNA methylation machinery*
**作者**：Wang Y et al.
**摘要**：利用重组ZBTB34蛋白进行体外结合实验，发现其与DNA甲基转移酶(DNMT3A)的关联，提示其在表观遗传修饰中的可能角色。
3. **文献名称**：*Expression and purification of recombinant human ZBTB34 in E. coli for structural studies*
**作者**：Chen H et al.
**摘要**：报道了ZBTB34在大肠杆菌中的重组表达及纯化方法，通过X射线晶体学初步解析了其锌指结构域的构象特征。
**备注**：若需获取具体文献，建议在PubMed、Google Scholar或领域数据库中以“ZBTB34 recombinant protein”“ZBTB34 function”为关键词检索最新研究。部分研究可能因该蛋白功能尚未完全明确而文献较少。
**Background of Recombinant Human ZBTB34 Protein**
ZBTB34. a member of the zinc finger and BTB domain-containing (ZBTB) protein family, is a transcription factor implicated in diverse cellular processes. Characterized by its N-terminal BTB/POZ domain and C-terminal zinc finger motifs, ZBTB34 is thought to regulate gene expression through protein-protein interactions and DNA binding. While its exact physiological role remains under investigation, ZBTB34 has been linked to transcriptional repression, chromatin remodeling, and cellular differentiation.
Studies suggest its involvement in development and disease. For example, ZBTB34 is expressed in various tissues, including the brain and immune system, hinting at roles in neural or immunological functions. Aberrant expression or mutations in ZBTB34 have been associated with cancers, such as gliomas and leukemias, where it may act as a tumor suppressor or oncogene depending on context.
Recombinant human ZBTB34 protein, produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cells), enables functional studies. Its purified form allows researchers to explore DNA-binding specificity, interaction partners, and mechanistic pathways. Current research aims to clarify its regulatory targets, post-translational modifications, and therapeutic potential in diseases linked to ZBTB34 dysregulation. This protein serves as a critical tool for unraveling its biological significance and translational applications.
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