| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB16 |
| Uniprot No | Q05516 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673 aa |
| 活性数据 | MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV |
| 分子量 | 74.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZBTB16蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *The PLZF gene of acute promyelocytic leukemia encodes a transcriptional repressor*
**作者**: Chen, Z., Brand, N.J., Chen, A. et al.
**摘要**: 该研究首次发现ZBTB16(PLZF)基因在急性早幼粒细胞白血病(APL)中的作用，揭示其编码的蛋白作为转录抑制因子，通过与RARA基因融合形成PLZF-RARA致癌融合蛋白，干扰细胞分化和增殖调控。
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2. **文献名称**: *Role of the zinc-finger protein ZBTB16 in hematopoietic stem cells*
**作者**: Costoya, J.A. et al.
**摘要**: 本研究发现ZBTB16对维持造血干细胞自我更新和多能性至关重要，其通过调控下游靶基因(如Hox家族)的表达参与干细胞命运决定，缺失ZBTB16会导致干细胞池耗竭。
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3. **文献名称**: *DNA-binding specificity of the recombinant ZBTB16 protein and its role in adipogenesis*
**作者**: Zhu, Q., et al.
**摘要**: 该研究利用重组ZBTB16蛋白揭示其通过结合特定DNA序列(如G-C rich区域)抑制脂肪生成基因表达，从而负调控脂肪细胞分化，为代谢疾病的治疗提供新靶点。
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以上文献涵盖了ZBTB16在白血病、干细胞调控及代谢中的关键功能研究。若需扩展，可查阅相关领域近年高影响力期刊如*Nature*或*Cell*的论文。
ZBTB16 (Zinc Finger and BTB Domain Containing 16), also known as promyelocytic leukemia zinc finger (PLZF), is a transcription factor encoded by the ZBTB16 gene in humans. It belongs to the BTB/POZ (Bric-a-brac, Tramtrack, Broad-Complex/Poxvirus and Zinc Finger) protein family, characterized by an N-terminal BTB domain involved in protein-protein interactions and C-terminal zinc finger motifs mediating DNA binding. Initially identified through its role in acute promyelocytic leukemia (APL), ZBTB16 forms fusion proteins with retinoic acid receptor alpha (RARα) due to chromosomal translocations, disrupting normal hematopoiesis.
Beyond its oncogenic associations, ZBTB16 plays critical roles in development, stem cell maintenance, and tissue homeostasis. It regulates cell differentiation, proliferation, and apoptosis across diverse biological systems, including hematopoiesis, spermatogenesis, and neuronal development. In stem cells, ZBTB16 acts as a pluripotency modulator, balancing self-renewal and lineage commitment. Its tumor-suppressive or oncogenic functions appear context-dependent, influenced by cellular environment and post-translational modifications.
Recombinant human ZBTB16 protein, typically produced in bacterial or mammalian expression systems, serves as a tool for studying molecular mechanisms, protein interactions, and therapeutic targeting. Researchers utilize it to explore its dual roles in cancer progression and suppression, as well as its potential in regenerative medicine and immune regulation.
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