| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR51B |
| Uniprot No | Q8TC44 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-478 aa |
| 活性数据 | MASATEDPVL ERYFKGHKAA ITSLDLSPNG KQLATASWDT FLMLWNFKPH ARAYRYVGHK DVVTSVQFSP HGNLLASASR DRTVRLWIPD KRGKFSEFKA HTAPVRSVDF SADGQFLATA SEDKSIKVWS MYRQRFLYSL YRHTHWVRCA KFSPDGRLIV SCSEDKTIKI WDTTNKQCVN NFSDSVGFAN FVDFNPSGTC IASAGSDQTV KVWDVRVNKL LQHYQVHSGG VNCISFHPSG NYLITASSDG TLKILDLLEG RLIYTLQGHT GPVFTVSFSK GGELFASGGA DTQVLLWRTN FDELHCKGLT KRNLKRLHFD SPPHLLDIYP RTPHPHEEKV ETVEINPKLE VIDLQISTPP VMDILSFDST TTTETSGRTL PDKGEEACGY FLNPSLMSPE CLPTTTKKKT EDMSDLPCES QRSIPLAVTD ALEHIMEQLN VLTQTVSILE QRLTLTEDKL KDCLENQQKL FSAVQQKS |
| 分子量 | 53.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR51B蛋白的参考文献示例(仅供参考，建议通过学术数据库核实具体文献)：
1. **文献名称**：*"WDR51B interacts with Rab33 to regulate autophagosome-lysosome fusion"*
**作者**：Chen L, et al.
**摘要**：本研究通过重组表达WDR51B蛋白，揭示了其通过与Rab33相互作用调控自噬小体与溶酶体融合的分子机制，为自噬失调相关疾病提供了潜在靶点。
2. **文献名称**：*"Structural characterization of recombinant human WDR51B and its role in mTOR signaling"*
**作者**：Smith JR, et al.
**摘要**：文章报道了重组WDR51B蛋白的晶体结构解析，并发现其通过调控mTOR通路影响细胞生长和代谢，为癌症治疗研究提供了新线索。
3. **文献名称**：*"Functional analysis of WDR51B in DNA damage repair using recombinant protein models"*
**作者**：Kim H, et al.
**摘要**：利用重组WDR51B蛋白进行体外实验，发现其参与DNA损伤修复过程，可能与同源重组修复通路中的关键蛋白形成复合物。
4. **文献名称**：*"WDR51B knockout and recombinant rescue experiments reveal its essential role in neuronal development"*
**作者**：Wang Q, et al.
**摘要**：通过重组WDR51B蛋白回补实验，证明其对神经元突触形成和发育至关重要，为神经退行性疾病机制研究提供了依据。
**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“WDR51B”或“ARAWP”检索。部分研究可能涉及WDR51B的别名或间接关联机制。
WDR51B (WD Repeat Domain 51B), also known as CCDC99 or WAFL, is a human protein belonging to the WD40 repeat family, characterized by conserved repeats of ~40 amino acids that form β-propeller structures to mediate protein-protein interactions. It is encoded by the WDR51B gene located on chromosome 12q13.12. While its precise biological role remains under investigation, WDR51B is implicated in intracellular trafficking and autophagy regulation. Studies suggest it interacts with components of the endosomal sorting complex required for transport (ESCRT) machinery, potentially influencing endosome-lysosome pathways or autophagosome formation.
WDR51B has been linked to cellular stress responses, with upregulated expression observed under nutrient deprivation, hinting at a role in adaptive survival mechanisms. Dysregulation of WDR51B may contribute to pathological conditions; for instance, aberrant expression is associated with cancers, including breast and liver cancers, where it might affect tumor progression through autophagy-related pathways. Its WD40 domains likely serve as scaffolds for assembling molecular complexes, though specific binding partners and signaling cascades remain poorly characterized.
Current research focuses on elucidating its structural motifs, interactome, and functional interplay with autophagy regulators like LC3 or ATG proteins. As a recombinant protein, WDR51B is utilized in biochemical assays to study interaction networks and validate genetic findings, aiding the exploration of its therapeutic potential in autophagy-related disorders. Further studies are needed to define its mechanistic contributions to cellular homeostasis and disease.
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