| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR13 |
| Uniprot No | Q9H1Z4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-485 aa |
| 活性数据 | MAAVWQQVLA VDARYNAYRT PTFPQFRTQY IRRRSQLLRE NAKAGHPPAL RRQYLRLRGQ LLGQRYGPLS EPGSARAYSN SIVRSSRTTL DRMEDFEDDP RALGARGHRR SVSRGSYQLQ AQMNRAVYED RPPGSVVPTS AAEASRAMAG DTSLSENYAF AGMYHVFDQH VDEAVPRVRF ANDDRHRLAC CSLDGSISLC QLVPAPPTVL RVLRGHTRGV SDFAWSLSND ILVSTSLDAT MRIWASEDGR CIREIPDPDS AELLCCTFQP VNNNLTVVGN AKHNVHVMNI STGKKVKGGS SKLTGRVLAL SFDAPGRLLW AGDDHGSVFS FLFDMATGKL TKAKRLVVHE GSPVTSISAR SWVSREARDP SLLINACLNK LLLYRVVDNE GTLQLKRSFP IEQSSHPVRS IFCPLMSFRQ GACVVTGSED MCVHFFDVER AAKAAVNKLQ GHSAPVLDVS FNCDESLLAS SDASGMVIVW RREQK |
| 分子量 | 53.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR13蛋白的参考文献示例，基于领域内常见研究方向整理：
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1. **《WDR13 regulates glycogen accumulation and neuronal functions in the developing brain》**
*作者：Swarup G, et al. (2019)*
**摘要**：研究利用重组人WDR13蛋白体外实验，发现其通过调控GSK3β活性影响神经元糖原代谢，并参与突触可塑性，提示其在中枢神经系统发育中的作用。
2. **《The X-linked intellectual disability gene WDR13 interacts with HDAC complexes to modulate transcriptional regulation》**
*作者：Li Y, et al. (2017)*
**摘要**：通过重组表达WDR13蛋白并结合质谱分析，鉴定其与组蛋白去乙酰化酶(HDAC)复合物的相互作用，揭示其在染色质修饰和智力障碍中的潜在机制。
3. **《Characterization of recombinant human WDR13 protein and its role in pancreatic β-cell function》**
*作者：Kumar R, et al. (2015)*
**摘要**：克隆并纯化重组人WDR13蛋白，证实其通过抑制细胞周期蛋白依赖性激酶(CDK4)调控β细胞增殖，可能参与糖尿病病理过程。
4. **《WDR13 deficiency promotes metabolic syndrome via mTORC1 signaling in mice》**
*作者：Singh K, et al. (2020)*
**摘要**：通过重组蛋白互补实验，验证WDR13缺失导致mTOR通路过度激活，进而引发肝脏脂质沉积和胰岛素抵抗，为代谢综合征提供分子机制解释。
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**说明**：以上为模拟参考文献，实际研究中请通过PubMed或Google Scholar等平台查询真实文献。研究关键词建议：**WDR13 AND (recombinant protein OR expression)**。
WDR13 (WD repeat-containing protein 13) is a conserved nuclear protein belonging to the WD40 repeat family, known for its role in mediating protein-protein interactions and regulating diverse cellular processes. The human WDR13 gene is located on the X chromosome (Xp11.23) and encodes a protein containing seven WD40 repeats, which form a characteristic β-propeller structure. This structural motif facilitates interactions with partner molecules, influencing transcriptional regulation, epigenetic modifications, and signaling pathways.
Studies suggest WDR13 is involved in cell cycle control, apoptosis, and autophagy. It interacts with transcriptional regulators, such as NF-κB and p53. modulating their activity to affect stress responses and cell survival. In mice, WDR13 deficiency leads to testicular abnormalities and metabolic imbalances, highlighting its importance in reproductive and metabolic homeostasis. In humans, altered WDR13 expression has been linked to cancers, neurological disorders, and metabolic diseases, though its exact mechanisms remain under investigation.
WDR13 is evolutionarily conserved across species, underscoring its fundamental biological role. Current research focuses on elucidating its molecular targets, involvement in disease pathways, and potential therapeutic applications. Its broad functional spectrum positions WDR13 as a critical, yet still enigmatic, player in cellular regulation and disease pathophysiology.
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