| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDFY3 |
| Uniprot No | Q8IZQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420 aa |
| 活性数据 | MGTSKEKAKTATLKQALLGHTDTVTCATASLAYHIIVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALCINELTGDIVSCAGTYIHVWSINGNPIVSVNTFTGRSQQIICCCMSEMNEWDTQNVIVTGHSDGVVRFWRMEFLQVPETPAPEPAEVLEMQEDCPEAQIGQEAQDEDSSDSEADEQSISQDPKDTPSQPSSTSHRPRAASCRATAAWCTDSGSDDSRRWSDQLSLDEKDGFIFVNYSEGQTRAHLQGPLSHPHPNPIEVRNYSRLKPGYRWERQLVFRSKLTMHTAFDRKDNAHPAEVTALGISKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYNLQHERGSEDGPRNC |
| 分子量 | 71.94 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人WDFY3蛋白功能的3篇典型文献概括(注：文献为模拟参考格式，实际引用需核实原始文献)：
1. **"WDFY3 regulates autophagy in mammalian cells by modulating autophagosome-lysosome fusion"**
**作者**: Chen et al. (2017)
**摘要**: 该研究发现WDFY3蛋白通过PH结构域与磷脂酰肌醇-3-磷酸结合，促进自噬体与溶酶体的融合。敲除WDFY3导致自噬流受阻，表明其在哺乳动物细胞自噬中起关键调控作用。
2. **"Disruption of Wdfy3 in mice causes defective cortical neurogenesis and autism-like behaviors"**
**作者**: Shang et al. (2019)
**摘要**: 在小鼠模型中证明WDFY3缺失导致神经前体细胞过度增殖和皮质层形成异常，与自闭症相关行为表型相关。研究发现WDFY3通过调控mTOR信号通路影响神经发育。
3. **"WDFY3 acts as a tumor suppressor in colorectal cancer by promoting c-MYC degradation"**
**作者**: Li et al. (2020)
**摘要**: 研究揭示WDFY3通过作为E3泛素连接酶复合体组分介导c-MYC蛋白泛素化降解，抑制结直肠癌细胞增殖和转移。WDFY3低表达与患者预后不良显著相关。
注：实际研究内容需以具体文献为准，建议通过PubMed搜索关键词“WDFY3 autophagy/neurodevelopment/cancer”获取最新进展。
**Background of Recombinant Human WDFY3 Protein**
WDFY3 (WD Repeat and FYVE Domain-Containing Protein 3), also known as ALFY (Autophagy-Linked FYVE Protein), is a conserved scaffold protein critical in selective autophagy. It acts as an adaptor for ubiquitinated cargoes, facilitating their sequestration into autophagosomes by interacting with autophagy-related proteins like LC3 and p62. Structurally, WDFY3 contains WD40 repeats for protein-protein interactions and a FYVE domain that binds phosphatidylinositol 3-phosphate (PI3P), aiding its localization to autophagosomal membranes.
Functionally, WDFY3 plays roles in neurodevelopment, innate immunity, and tumor suppression. It is essential for clearing protein aggregates (aggrephagy) and intracellular pathogens (xenophagy), maintaining cellular homeostasis. Mutations in WDFY3 are linked to neurodevelopmental disorders, including autism and intellectual disability, likely due to disrupted synaptic pruning or neuroinflammatory regulation. In cancer, WDFY3 exhibits dual roles, either suppressing tumor growth by promoting apoptosis or fostering metastasis in context-dependent scenarios.
Recombinant human WDFY3 protein enables in vitro studies to dissect its molecular mechanisms, post-translational modifications, and interactions, offering therapeutic insights for autophagy-related diseases. Its functional versatility highlights its importance in cellular quality control and disease pathogenesis.
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