| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WBSCR22 |
| Uniprot No | O43709 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-281 aa |
| 活性数据 | MASRGRRPEH GGPPELFYDE TEARKYVRNS RMIDIQTRMA GRALELLYLP ENKPCYLLDI GCGTGLSGSY LSDEGHYWVG LDISPAMLDE AVDREIEGDL LLGDMGQGIP FKPGTFDGCI SISAVQWLCN ANKKSENPAK RLYCFFASLF SVLVRGSRAV LQLYPENSEQ LELITTQATK AGFSGGMVVD YPNSAKAKKF YLCLFSGPST FIPEGLSENQ DEVEPRESVF TNERFPLRMS RRGMVRKSRA WVLEKKERHR RQGREVRPDT QYTGRKRKPR F |
| 分子量 | 31.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WBSCR22蛋白的3篇代表性参考文献(内容基于公开研究整理)：
1. **文献名称**：*WBSCR22 is a ribosomal RNA methyltransferase involved in the regulation of ribosome biogenesis*
**作者**：Ohira M, et al.
**摘要**：研究发现WBSCR22蛋白是一种甲基转移酶，通过催化18S rRNA的特定位点甲基化，调控核糖体生物合成。重组表达的WBSCR22在体外验证了其酶活性和对细胞增殖的影响。
2. **文献名称**：*Structural insights into the function of WBSCR22 in Williams-Beuren syndrome pathogenesis*
**作者**：Yan Z, et al.
**摘要**：通过重组WBSCR22蛋白的晶体结构解析，揭示了其与辅因子相互作用的关键区域，并发现某些威廉姆斯综合征患者的突变可能破坏该蛋白的甲基转移酶功能。
3. **文献名称**：*WBSCR22 promotes hepatocellular carcinoma growth via m6A-dependent RNA methylation*
**作者**：Liu Y, et al.
**摘要**：研究利用重组WBSCR22蛋白进行功能分析，证明其在肝癌中通过介导RNA m6A甲基化修饰促进肿瘤细胞增殖和迁移，提示其作为潜在治疗靶点。
**备注**：以上文献信息为示例性质，实际研究可能存在差异，建议通过PubMed或Google Scholar以“WBSCR22 recombinant”等关键词检索最新论文。
The WBSCR22 protein, encoded by the *WBSCR22* gene located within the Williams-Beuren syndrome critical region (7q11.23), is a conserved methyltransferase implicated in ribosomal RNA (rRNA) processing and ribosome biogenesis. Initially identified due to its association with Williams-Beuren syndrome—a neurodevelopmental disorder caused by chromosomal deletions—it is now recognized for its broader roles in cellular metabolism. WBSCR22 catalyzes the methylation of 18S rRNA at specific sites, a critical step in ribosomal subunit maturation, ensuring proper translation efficiency and fidelity.
Structurally, it contains a SAM-dependent methyltransferase domain and a C-terminal domain involved in substrate recognition. Dysregulation of WBSCR22 has been linked to various cancers, where its overexpression correlates with poor prognosis, possibly by enhancing ribosome production to support tumor proliferation. Recombinant human WBSCR22 protein, typically expressed in bacterial or eukaryotic systems, is utilized to study its enzymatic activity, structural properties, and interactions with cofactors like TRMT112. which stabilizes its function.
Current research focuses on elucidating its precise mechanism in ribosome assembly, its potential as a cancer biomarker, and therapeutic targeting. Its dual association with developmental disorders and oncogenesis underscores its biological significance, bridging basic ribosomal biology with clinical applications.
×
