| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WBSCR28 |
| Uniprot No | Q6UE05 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-265 aa |
| 活性数据 | MEALPPVRSSLLGNLLQVTRLSVLLVQNRDHLYNFLLLKINLFNHWVSGLAQEARGSCNWQAHLPLGAADCPLGQALRAGLALIQVPVWLVLQGPRLMWAGMWGSTKGLGLALLSAWEQLGLSVAIWTDLFLSCLHGLMLVALLLVVVTWRVCQKSHCFRLGRQLSKALQVNCVVRKLLVQLRRLYWWVETMTALTSWHLAYLITWTTCLASHLLQAAFEHTTQLAEAQEVEPQEVSGSSLLPSLSASSDSESGTVLPEQETPRE |
| 分子量 | 55.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WBSCR28蛋白的模拟参考文献示例(由于该蛋白的研究可能较少或存在别名，实际文献需通过专业数据库验证)：
1. **文献名称**：*Functional characterization of the WBSCR28 protein in chromatin remodeling*
**作者**：Smith A, et al.
**摘要**：研究通过重组表达WBSCR28蛋白，发现其参与染色质结构调控，并可能与威廉姆斯综合征的致病机制相关。
2. **文献名称**：*Structural analysis of recombinant human WBSCR28 and its interaction partners*
**作者**：Lee B, et al.
**摘要**：利用X射线晶体学解析WBSCR28蛋白结构，揭示其与转录因子TFII-I的相互作用，提示其在基因表达调控中的潜在作用。
3. **文献名称**：*WBSCR28 knockout models reveal its role in cardiovascular development*
**作者**：Garcia R, et al.
**摘要**：通过重组蛋白功能回补实验，验证WBSCR28在小鼠模型中对心血管发育的影响，支持其在威廉姆斯综合征表型中的作用。
**提示**：WBSCR28可能与威廉姆斯综合征相关基因簇(如WBSCR区域)有关，建议检索时结合关键词"Williams-Beuren syndrome"或"chromosome 7q11.23"。实际文献可能较少，需关注其别名(如某些研究中可能归类为泛素连接酶或转录辅因子)。
The Williams-Beuren syndrome chromosomal region 28 protein (WBSCR28), also known as BAZ1B or WSTF, is a chromatin-remodeling factor encoded within the 7q11.23 chromosomal region, which is associated with Williams-Beuren syndrome (WBS), a neurodevelopmental disorder. As a member of the SNF2 family of ATP-dependent chromatin remodelers, WBSCR28 plays critical roles in transcription regulation, DNA repair, and replication. It functions as a core component of the ISWI (Imitation Switch) chromatin-remodeling complexes, particularly in the WICH complex (WSTF-ISWI chromatin-remodeling complex), where it interacts with SNF2H to modulate nucleosome spacing and histone dynamics.
Recombinant WBSCR28 protein is commonly expressed in eukaryotic systems to preserve post-translational modifications and functional interactions. Studies utilize it to investigate its role in chromatin structure maintenance, histone acetylation modulation (e.g., H2BK120 acetylation), and its association with WBS phenotypes like cardiovascular defects and cognitive impairments. Its biochemical activity, including ATP hydrolysis and nucleosome binding, is frequently analyzed to elucidate mechanisms underlying transcriptional dysregulation in WBS and related disorders. This protein serves as a vital tool for dissecting chromatin biology and disease pathophysiology.
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