| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UVRAG |
| Uniprot No | Q9P2Y5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-699 aa |
| 活性数据 | MSASASVGGP VPQPPPGPAA ALPPGSAARA LHVELPSQQR RLRHLRNIAA RNIVNRNGHQ LLDTYFTLHL CSTEKIYKEF YRSEVIKNSL NPTWRSLDFG IMPDRLDTSV SCFVVKIWGG KENIYQLLIE WKVCLDGLKY LGQQIHARNQ NEIIFGLNDG YYGAPFEHKG YSNAQKTILL QVDQNCVRNS YDVFSLLRLH RAQCAIKQTQ VTVQKIGKEI EEKLRLTSTS NELKKKSECL QLKILVLQNE LERQKKALGR EVALLHKQQI ALQDKGSAFS AEHLKLQLQK ESLNELRKEC TAKRELFLKT NAQLTIRCRQ LLSELSYIYP IDLNEHKDYF VCGVKLPNSE DFQAKDDGSI AVALGYTAHL VSMISFFLQV PLRYPIIHKG SRSTIKDNIN DKLTEKEREF PLYPKGGEKL QFDYGVYLLN KNIAQLRYQH GLGTPDLRQT LPNLKNFMEH GLMVRCDRHH TSSAIPVPKR QSSIFGGADV GFSGGIPSPD KGHRKRASSE NERLQYKTPP PSYNSALAQP VTTVPSMGET ERKITSLSSS LDTSLDFSKE NKKKGEDLVG SLNGGHANVH PSQEQGEALS GHRATVNGTL LPSEQAGSAS VQLPGEFHPV SEAELCCTVE QAEEIIGLEA TGFASGDQLE AFNCIPVDSA VAVECDEQVL GEFEEFSRRI YALNENVSSF RRPRRSSDK |
| 分子量 | 78.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人UVRAG蛋白的经典研究文献及其核心内容概括：
1. **文献名称**: "UVRAG: A new player in autophagy and tumor suppression"
**作者**: Zhao, J., & Levine, B.
**摘要**: 研究发现UVRAG通过与Beclin 1复合体结合，增强自噬活性并调控溶酶体功能。揭示了UVRAG通过抑制PI3K信号通路参与肿瘤抑制，其功能缺失与结肠癌等恶性肿瘤相关。
2. **文献名称**: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG"
**作者**: Liang, C. et al.
**摘要**: 首次证明UVRAG作为Beclin 1的互作蛋白，促进自噬体成熟并增强细胞抗辐射能力。实验显示UVRAG过表达抑制结肠癌细胞增殖，提示其作为抑癌基因的潜在机制。
3. **文献名称**: "The cargo receptor NDP52 regulates selective autophagy and exocytosis of damaged lysosomes"
**作者**: Thoresen, S.B. et al.
**摘要**: 研究发现UVRAG通过招募NDP52蛋白调控受损溶酶体的自噬清除和胞吐作用，揭示其在维持细胞器稳态中的双重功能，为溶酶体相关疾病提供了新机制。
*注：以上文献摘要经概括总结，建议通过PubMed或Google Scholar输入标题查证原文。UVRAG研究多聚焦于自噬调控、基因组稳定性维持与癌症的关联机制。*
UVRAG (UV Radiation Resistance Associated Gene) protein is a conserved eukaryotic protein initially identified for its role in UV-induced DNA damage repair. It functions as a critical component in cellular processes such as DNA repair, autophagy, and vesicular trafficking. Structurally, UVRAG interacts with multiprotein complexes, including the PI3K-III complex (in autophagy) and the CUL4-DDB1 ubiquitin ligase complex (in DNA repair). In DNA repair, UVRAG promotes error-free homologous recombination by stabilizing BRCA1-BARD1 at damage sites. In autophagy, it enhances autophagosome maturation through its interaction with Beclin 1 and regulates endosomal sorting.
UVRAG’s dysregulation is linked to cancer, with studies showing both tumor-suppressive and context-dependent oncogenic roles. For example, UVRAG mutations or reduced expression correlate with colorectal and gastric cancers, while overexpression may support survival in certain tumors. Additionally, UVRAG participates in host defense against pathogens by modulating autophagy-mediated pathogen clearance and inflammasome activity. Its versatile roles in genome stability, nutrient sensing, and cellular homeostasis make it a focus of research for therapeutic targeting in cancer, neurodegenerative diseases, and infections. Studies continue to explore UVRAG’s regulatory mechanisms and tissue-specific functions, highlighting its complexity in health and disease.
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