| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UTP18 |
| Uniprot No | Q9Y5J1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-556 aa |
| 活性数据 | MPPERRRRMK LDRRTGAKPK RKPGMRPDWK AGAGPGGPPQ KPAPSSQRKP PARPSAAAAA IAVAAAEEER RLRQRNRLRL EEDKPAVERC LEELVFGDVE NDEDALLRRL RGPRVQEHED SGDSEVENEA KGNFPPQKKP VWVDEEDEDE EMVDMMNNRF RKDMMKNASE SKLSKDNLKK RLKEEFQHAM GGVPAWAETT KRKTSSDDES EEDEDDLLQR TGNFISTSTS LPRGILKMKN CQHANAERPT VARISSVQFH PGAQIVMVAG LDNAVSLFQV DGKTNPKIQS IYLERFPIFK ACFSANGEEV LATSTHSKVL YVYDMLAGKL IPVHQVRGLK EKIVRSFEVS PDGSFLLING IAGYLHLLAM KTKELIGSMK INGRVAASTF SSDSKKVYAS SGDGEVYVWD VNSRKCLNRF VDEGSLYGLS IATSRNGQYV ACGSNCGVVN IYNQDSCLQE TNPKPIKAIM NLVTGVTSLT FNPTTEILAI ASEKMKEAVR LVHLPSCTVF SNFPVIKNKN ISHVHTMDFS PRSGYFALGN EKGKALMYRL HHYSDF |
| 分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UTP18蛋白的模拟参考文献示例(仅供学习参考，非真实文献)：
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1. **文献名称**: "Expression and Structural Analysis of Recombinant Human UTP18 in Ribosome Biogenesis"
**作者**: Zhang L. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人UTP18蛋白，通过X射线晶体学解析其三维结构，揭示其与核糖体RNA加工复合体的相互作用机制。
2. **文献名称**: "UTP18 Knockdown Impairs Cell Proliferation via rRNA Processing Defects in Colon Cancer Cells"
**作者**: Chen Y. et al.
**摘要**: 利用重组UTP18蛋白功能回补实验，证实UTP18通过调控18S rRNA成熟影响结肠癌细胞增殖，为癌症靶向治疗提供新方向。
3. **文献名称**: "UTP18 Interacts with DEAD-box RNA Helicase DDX21 to Modulate Neuronal Differentiation"
**作者**: Wang Q. et al.
**摘要**: 通过重组蛋白体外结合实验，发现UTP18与DDX21形成复合物，参与调控神经元分化中的核糖体组装及mRNA翻译效率。
4. **文献名称**: "A High-Throughput Screen Identifies UTP18 as a Potential Regulator of p53 Stability"
**作者**: Kumar S. et al.
**摘要**: 研究利用重组UTP18蛋白及CRISPR筛选技术，发现其通过泛素-蛋白酶体通路调控p53蛋白稳定性，影响DNA损伤应答。
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*注：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)查询。建议结合具体研究方向补充真实文献。*
UTP18 (U3 Small Nucleolar RNA-Associated Protein 18 Homolog) is a conserved nucleolar protein involved in the early stages of ribosomal RNA (rRNA) processing. As a component of the U3 small nucleolar ribonucleoprotein (snoRNP) complex, it plays a critical role in 18S rRNA maturation within the small ribosomal subunit (SSU) processome. Structurally, UTP18 contains WD40 repeat domains, facilitating protein-protein interactions essential for the assembly and stability of the SSU processome.
Recombinant human UTP18 protein is typically produced using engineered expression systems (e.g., E. coli, yeast, or mammalian cells) to ensure proper post-translational modifications. Its recombinant form enables detailed biochemical and functional studies, including interactions with other processome components like UTP14A and RRP7A. Researchers utilize purified UTP18 to dissect ribosome biogenesis mechanisms, which are fundamental to cellular growth and proliferation. Dysregulation of UTP18 has been implicated in ribosomopathies and cancer, where aberrant ribosome synthesis contributes to pathological states. Studies also explore its role in developmental disorders linked to rRNA processing defects. Recombinant UTP18 serves as a vital tool for structural analysis (e.g., cryo-EM), drug screening targeting ribosome biosynthesis pathways, and understanding cell cycle-dependent regulation of nucleolar activities. Its conserved function across eukaryotes underscores its evolutionary importance in ribosome assembly.
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