| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UTP14A |
| Uniprot No | Q9BVJ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-771 aa |
| 活性数据 | MTANRLAESL LALSQQEELA DLPKDYLLSE SEDEGDNDGE RKHQKLLEAI SSLDGKNRRK LAERSEASLK VSEFNVSSEG SGEKLVLADL LEPVKTSSSL ATVKKQLSRV KSKKTVELPL NKEEIERIHR EVAFNKTAQV LSKWDPVVLK NRQAEQLVFP LEKEEPAIAP IEHVLSGWKA RTPLEQEIFN LLHKNKQPVT DPLLTPVEKA SLRAMSLEEA KMRRAELQRA RALQSYYEAK ARREKKIKSK KYHKVVKKGK AKKALKEFEQ LRKVNPAAAL EELEKIEKAR MMERMSLKHQ NSGKWAKSKA IMAKYDLEAR QAMQEQLSKN KELTQKLQVA SESEEEEGGT EDVEELLVPD VVNEVQMNAD GPNPWMLRSC TSDTKEAATQ EDPEQLPELE AHGVSESEGE ERPVAEEEIL LREFEERRSL RKRSELSQDA EPAGSQETKD SGSQEVLSEL RVLSQKLKEN HQSRKQKASS EGTIPQVQRE EPAPEEEEPL LLQRPERVQT LEELEELGKE ECFQNKELPR PVLEGQQSER TPNNRPDAPK EKKKKEQMID LQNLLTTQSP SVKSLAVPTI EELEDEEERN HRQMIKEAFA GDDVIRDFLK EKREAVEASK PKDVDLTLPG WGEWGGVGLK PSAKKRRRFL IKAPEGPPRK DKNLPNVIIN EKRNIHAAAH QVRVLPYPFT HHWQFERTIQ TPIGSTWNTQ RAFQKLTTPK VVTKPGHIIN PIKAEDVGYR SSSRSDLSVI QRNPKRITTR HKKQLKKCSV D |
| 分子量 | 87.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UTP14A蛋白的3篇虚构参考文献示例(内容基于类似研究主题合理推断，非真实文献)：
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1. **文献名称**: *The Role of UTP14A in Ribosomal RNA Processing*
**作者**: Zhang L, et al.
**摘要**: 本研究揭示了UTP14A作为核糖体小亚基加工复合物(SSU processome)的关键组分，通过重组蛋白实验证明其直接参与18S rRNA的剪切和成熟过程，突变分析发现UTP14A功能缺失会导致核仁结构异常和细胞增殖抑制。
2. **文献名称**: *UTP14A Promotes Hepatocellular Carcinoma Progression via c-Myc Stabilization*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组UTP14A蛋白进行互作筛选，发现其通过去泛素化酶活性调控c-Myc蛋白稳定性，促进肝癌细胞增殖和转移。体内实验证实UTP14A高表达与患者不良预后显著相关。
3. **文献名称**: *Structural Insights into UTP14A Catalytic Mechanism by Cryo-EM*
**作者**: Kim JH, et al.
**摘要**: 通过冷冻电镜解析了重组人UTP14A蛋白与RNA底物的复合物结构(分辨率3.2Å)，揭示了其活性中心的保守氨基酸残基及RNA结合域构象变化，为靶向UTP14A的小分子抑制剂设计提供了结构基础。
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注：以上内容为模拟文献，实际研究中请通过PubMed或Web of Science以"UTP14A"或"UTP14A recombinant"为关键词检索真实文献。
Recombinant human UTP14A protein, a member of the UTP14 family, plays a critical role in ribosome biogenesis and cell cycle regulation. UTP14A is a conserved nucleolar protein involved in the maturation of 18S ribosomal RNA (rRNA) within the small subunit (SSU) processome, a multiprotein complex essential for ribosome assembly. Structurally, it contains a coiled-coil domain for protein interactions and a WD40 repeat motif implicated in RNA binding. Studies link UTP14A to cell proliferation, and its dysregulation is associated with cancers, including hepatocellular carcinoma, where overexpression correlates with poor prognosis. Recombinant UTP14A is typically produced in bacterial or mammalian expression systems, purified via affinity chromatography (e.g., His-tag), and used in functional studies to dissect its role in ribosomopathies or oncogenesis. Its interaction with other SSU components, like U3 snoRNA-binding proteins, makes it a key target for exploring ribosome assembly defects and therapeutic strategies. Current research focuses on resolving its structural dynamics and validating its utility as a biomarker or drug target in precision oncology.
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