| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UTP6 |
| Uniprot No | Q9NYH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-597 aa |
| 活性数据 | AEIIQERIE DRLPELEQLE RIGLFSHAEI KAIIKKASDL EYKIQRRTLF KEDFINYVQY EINLLELIQR RRTRIGYSFK KDEIENSIVH RVQGVFQRAS AKWKDDVQLW LSYVAFCKKW ATKTRLSKVF SAMLAIHSNK PALWIMAAKW EMEDRLSSES ARQLFLRALR FHPECPKLYK EYFRMELMHA EKLRKEKEEF EKASMDVENP DYSEEILKGE LAWIIYKNSV SIIKGAEFHV SLLSIAQLFD FAKDLQKEIY DDLQALHTDD PLTWDYVARR ELEIESQTEE QPTTKQAKAV EVGRKEERCC AVYEEAVKTL PTEAMWKCYI TFCLERFTKK SNSGFLRGKR LERTMTVFRK AHELKLLSEC QYKQLSVSLL CYNFLREALE VAVAGTELFR DSGTMWQLKL QVLIESKSPD IAMLFEEAFV HLKPQVCLPL WISWAEWSEG AKSQEDTEAV FKKALLAVIG ADSVTLKNKY LDWAYRSGGY KKARAVFKSL QESRPFSVDF FRKMIQFEKE QESCNMANIR EYYERALREF GSADSDLWMD YMKEELNHPL GRPENCGQIY WRAMKMLQGE SAEAFVAKHA MHQTGHL |
| 分子量 | 70.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人UTP6蛋白的参考文献示例(内容为模拟虚构，供参考)：
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1. **文献名称**: *Functional Characterization of Recombinant Human UTP6 in 18S rRNA Processing*
**作者**: Chen L, et al. (2018)
**摘要**: 本研究通过大肠杆菌表达系统成功纯化重组人UTP6蛋白，并证明其在体外可与UTP复合体(UTP-C)其他亚基结合。RNA干扰实验显示UTP6缺失导致18S rRNA前体加工受阻，提示其在核糖体小亚基成熟中的关键作用。
2. **文献名称**: *Cryo-EM Structure of the Human UTP Complex Reveals UTP6's Role in Structural Stability*
**作者**: Müller S, et al. (2020)
**摘要**: 通过冷冻电镜解析UTP-C复合体的高分辨率结构，发现UTP6作为核心支架蛋白与UTP4、UTP5相互作用维持复合体构象。重组UTP6的突变体实验表明其C端结构域对复合体组装及rRNA加工活性不可或缺。
3. **文献名称**: *UTP6 Dysregulation Linked to Impaired Ribosome Biogenesis in Myelodysplastic Syndromes*
**作者**: Wang Y, et al. (2021)
**摘要**: 分析骨髓增生异常综合征患者的基因表达数据发现，UTP6表达显著下调。体外过表达重组UTP6可部分恢复患者细胞中18S rRNA加工缺陷，提示其在疾病中可能作为治疗靶点。
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注：以上文献为假设性示例，实际研究请参考真实数据库(如PubMed)文献检索。
Recombinant human UTP6 protein, also known as small subunit processome component UTP6 or C1orf22. is a key player in ribosome biogenesis. As a core component of the U3 small nucleolar ribonucleoprotein (snoRNP) complex, it contributes to the early stages of 18S ribosomal RNA (rRNA) processing within the nucleolus. UTP6 is part of the t-UTP subcomplex, which facilitates transcription-coupled RNA modifications and rRNA folding. This 78 kDa protein contains conserved HEAT repeats that mediate protein-protein interactions critical for assembling the small ribosomal subunit.
The recombinant form is typically produced using prokaryotic (e.g., E. coli) or eukaryotic expression systems, enabling controlled studies of its molecular functions. Researchers utilize recombinant UTP6 to investigate ribosomopathy disorders, as defective rRNA processing has been linked to developmental conditions like Bowen-Conradi syndrome and cancer progression. Its role in cell proliferation makes it a potential biomarker in malignancies with upregulated ribosome synthesis.
Structural studies using recombinant proteins have helped map interaction domains with other processome components like UTP4 and UTP14. Current research focuses on elucidating UTP6's precise mechanistic contributions to ribosome maturation and exploring therapeutic targeting opportunities in diseases involving dysregulated ribosome biogenesis. Conservation across eukaryotes underscores its fundamental biological significance.
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