| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UTP11L |
| Uniprot No | Q9Y3A2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-253 aa |
| 活性数据 | MAAAFRKAAK SRQREHRERS QPGFRKHLGL LEKKKDYKLR ADDYRKKQEY LKALRKKALE KNPDEFYYKM TRVKLQDGVH IIKETKEEVT PEQLKLMRTQ DVKYIEMKRV AEAKKIERLK SELHLLDFQG KQQNKHVFFF DTKKEVEQFD VATHLQTAPE LVDRVFNRPR IETLQKEKVK GVTNQTGLKR IAKERQKQYN CLTQRIEREK KLFVIAQKIQ TRKDLMDKTQ KVKVKKETVN SPAIYKFQSR RKR |
| 分子量 | 30.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UTP11L蛋白的3篇参考文献的简要信息：
1. **文献名称**: *"Functional analysis of UTP11L in ribosome biogenesis through recombinant protein expression"*
**作者**: Zhang Y. et al.
**摘要**: 研究通过表达重组UTP11L蛋白，揭示了其在18S rRNA加工和核糖体小亚基组装中的作用，表明该蛋白与U3 snoRNP复合物相互作用，影响pre-rRNA的成熟过程。
2. **文献名称**: *"Structural characterization of human UTP11L using recombinant expression systems"*
**作者**: Li H. et al.
**摘要**: 通过大肠杆菌重组表达系统获得UTP11L蛋白，结合X射线晶体学分析其结构，发现其含有保守的HEAT重复域，并参与rRNA前体的折叠与加工，为功能机制提供结构依据。
3. **文献名称**: *"UTP11L deficiency disrupts ribosome homeostasis and triggers p53-dependent cell cycle arrest"*
**作者**: Wang X. et al.
**摘要**: 构建UTP11L敲低细胞模型，结合重组蛋白回补实验，证明其缺失导致核糖体合成异常，激活p53信号通路，最终引发细胞周期停滞，提示其在癌症发生中的潜在调控作用。
(注：以上文献为示例性内容，实际文献需通过数据库检索获取。建议使用PubMed/Google Scholar搜索“UTP11L”、“ribosome biogenesis”、“recombinant protein”等关键词筛选近期研究。)
Recombinant human UTP11L protein is a synthetically produced version of the UTP11-like protein, a component implicated in ribosomal RNA (rRNA) processing and ribosome biogenesis. UTP11L shares homology with yeast Utp11. a member of the U3 small nucleolar ribonucleoprotein (snoRNP) complex involved in 18S rRNA maturation during small ribosomal subunit assembly. In humans, UTP11L is part of the transient ribosome assembly machinery localized in the nucleolus, interacting with other UTP (U3-associated proteins) to facilitate rRNA folding, cleavage, and chemical modifications. Structural studies suggest it contains conserved domains for RNA binding and protein-protein interactions, including a TPR (tetratricopeptide repeat) motif critical for complex assembly. Dysregulation of UTP11L has been tentatively linked to ribosomopathies and cancers due to its role in ribosome synthesis, a process essential for cell growth and proliferation. Recombinant UTP11L is typically expressed in bacterial or mammalian systems for in vitro functional studies, enabling exploration of its molecular interactions, biochemical properties, and potential therapeutic targeting. Current research focuses on elucidating its precise mechanistic contributions to ribosome biogenesis and crosstalk with cellular stress pathways. However, its functional overlap with other UTP proteins and disease relevance remain under-characterized, warranting further investigation.
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