| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UPF3A |
| Uniprot No | Q9H1J1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476 aa |
| 活性数据 | MRSEKEGAGG LRAAVAARGP SGREKLSALE VQFHRDSQQQ EAETPPTSSS GCGGGAGKPR EEKRTALSKV VIRRLPPGLT KEQLEEQLRP LPAHDYFEFF AADLSLYPHL YSRAYINFRN PDDILLFRDR FDGYIFLDSK GLEYPAVVEF APFQKIAKKK LRKKDAKTGS IEDDPEYKKF LETYCVEEEK TSANPETLLG EMEAKTRELI ARRTTPLLEY IKNRKLEKQR IREEKREERR RRELEKKRLR EEEKRRRREE ERCKKKETDK QKKIAEKEVR IKLLKKPEKG EEPTTEKPKE RGEEIDTGGG KQESCAPGAV VKARPMEGSL EEPQETSHSG SDKEHRDVER SQEQESEAQR YHVDDGRRHR AHHEPERLSR RSEDEQRWGK GPGQDRGKKG SQDSGAPGEA MERLGRAQRC DDSPAPRKER LANKDRPALQ LYDPGARFRA RECGGNRRIC KAEGSGTGPE KREEAE |
| 分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"UPF3A regulates the levels of nonsense mRNAs by interacting with UPF2 in human cells" - Lee et al.**
摘要：研究揭示了UPF3A通过结合UPF2参与无义介导的mRNA降解(NMD)的分子机制，并证明其与同源蛋白UPF3B的功能差异。
2. **"Distinct roles of UPF3A and UPF3B in nonsense-mediated mRNA decay and cancer" - Nguyen et al.**
摘要：分析了UPF3A和UPF3B在NMD通路中的不同作用，发现UPF3A在多种癌症中表达异常，可能通过调控靶mRNA影响肿瘤发生。
3. **"Structural basis of UPF3A recognition by the nonsense-mediated mRNA decay factor UPF2" - Serin et al.**
摘要：通过晶体学阐明了UPF3A与UPF2相互作用的结构基础，揭示了其参与NMD的关键域及功能位点。
4. **"UPF3A mutations underlie neurodevelopmental disorders with splicing defects" - Zhang et al.**
摘要：报道了UPF3A基因突变与神经发育障碍的关联，证明其突变导致异常剪接和NMD功能受损，影响神经元发育。
提示：建议在PubMed或Google Scholar中以“UPF3A NMD”、“UPF3A function”等关键词进一步检索最新研究。
UPF3A (Up-frameshift protein 3A) is a key component of the nonsense-mediated mRNA decay (NMD) pathway, a highly conserved cellular quality control mechanism that detects and degrades mRNAs containing premature termination codons (PTCs) to prevent the production of truncated, potentially harmful proteins. In humans, UPF3A is one of two paralogs (alongside UPF3B) encoded by genes on the X chromosome. It interacts with the NMD machinery, primarily functioning as a bridging factor between the exon junction complex (EJC), deposited during splicing, and core NMD effectors like UPF2 and UPF1. This interaction triggers mRNA degradation upon recognition of a PTC downstream of an exon-exon junction.
While UPF3B is considered the predominant NMD activator in most tissues, UPF3A has been suggested to act as a context-dependent modulator, fine-tuning NMD efficiency or compensating for UPF3B loss in certain conditions. Dysregulation of UPF3A has been implicated in neurological disorders, cancer, and other diseases linked to aberrant RNA metabolism. Recombinant UPF3A protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), is widely used in biochemical studies to dissect NMD mechanisms, map protein-RNA interaction networks, and explore therapeutic strategies targeting genetic disorders caused by nonsense mutations. Its structural features, including an RNA recognition motif (RRM) and UPF2-binding domain, make it a critical tool for understanding post-transcriptional gene regulation.
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