| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNQ846 |
| Uniprot No | Q6UWW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146 aa |
| 活性数据 | MSRSRLFSVTSAISTIGILCLPLFQLVLSDLPCEEDEMCVNYNDQHPNGWYIWILLLLVLVAALLCGAVVLCLQCWLRRPRIDSHRRTMAVFAVGDLDSIYGTEAAVSPTVGIHLQTQTPDLYPVPAPCFGPLGSPPPYEEIVKTT |
| 分子量 | 42.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是根据学术数据库整理的重组人UNQ846蛋白相关文献的简要示例(注：UNQ846研究可能较少，以下内容为模拟示例)：
1. **文献名称**: *Functional characterization of recombinant human UNQ846 in cellular proliferation*
**作者**: Li X, et al. (2021)
**摘要**: 本研究通过大肠杆菌系统重组表达并纯化UNQ846蛋白，体外实验表明该蛋白通过调控MAPK通路显著促进内皮细胞增殖，可能参与血管生成。
2. **文献名称**: *Structural analysis of UNQ846 reveals a novel zinc-binding motif*
**作者**: Garcia-Ruiz E, et al. (2019)
**摘要**: 利用X射线晶体学解析UNQ846的三维结构，发现其N端存在新型锌指结构域，提示在DNA修复中可能发挥作用，重组蛋白在体外显示核酸酶活性。
3. **文献名称**: *UNQ846 as a potential biomarker in colorectal cancer*
**作者**: Wang T, et al. (2022)
**摘要**: 通过重组UNQ846蛋白制备单克隆抗体，临床样本分析显示该蛋白在结直肠癌组织中高表达，与患者生存率负相关，可能作为诊疗靶点。
4. **文献名称**: *Expression optimization of recombinant UNQ846 in mammalian systems*
**作者**: Müller S, et al. (2020)
**摘要**: 比较昆虫细胞/HEK293系统中UNQ846的表达效率，发现哺乳动物系统分泌的蛋白糖基化修饰更完整，功能活性提高2.3倍。
**注**：上述内容为模拟文献，实际研究中UNQ846(C10orf99)的相关研究较少，建议通过UniProt或PubMed使用编号/别名(如C10orf99)扩展检索。
**Background of Recombinant Human UNQ846 Protein**
The recombinant human UNQ846 protein, also designated as C10orf99 (Chromosome 10 open reading frame 99), is a small, secreted protein encoded by the *C10orf99* gene. It is classified as a member of the "uncharacterized protein" family due to limited structural and functional insights. Bioinformatic analyses predict UNQ846 to possess a conserved signal peptide, suggesting its secretion into extracellular spaces, along with coiled-coil domains and potential N-glycosylation sites, which may contribute to protein-protein interactions or structural stability.
Expression studies indicate that UNQ846 is predominantly found in placental tissue, prostate, and salivary glands, with lower levels detected in other organs. Its restricted expression hints at tissue-specific roles, potentially in reproductive or secretory functions. Although its precise biological mechanism remains unclear, preliminary studies suggest involvement in cellular adhesion, immune modulation, or signaling pathways, possibly linked to extracellular matrix regulation.
Research on recombinant UNQ846 focuses on elucidating its interaction partners and physiological relevance. Its recombinant form, produced via bacterial or mammalian expression systems, enables antibody development, functional assays, and exploration of its role in pathologies such as cancer or inflammatory conditions. Despite progress, UNQ846 remains understudied, representing a novel target for decoding its contribution to human biology and disease.
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