| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNR |
| Uniprot No | O75534 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-798 aa |
| 活性数据 | MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERMNGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMKEAFGFIERGDVVKEIFFHYSEFKGDLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVRMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQVATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQTEYQGMIEIVEEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID |
| 分子量 | 93.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
### 关于重组人UNR蛋白的参考文献(简要版)：
1. **"UNR interacts with the 5' untranslated region of the enterovirus genomic RNA to control viral translation"**
- **作者**: *Wurth L, Papasaikas P, Olmeda D, et al.*
- **摘要**: 研究重组人UNR蛋白的RNA结合能力，揭示其通过结合肠道病毒RNA的5'UTR调控病毒翻译的分子机制。实验使用重组UNR证明其作为RNA伴侣的功能。
2. **"Structural and functional analysis of the human UNR as an RNA-binding protein"**
- **作者**: *Tripsianes K, Madl T, Machyna M, et al.*
- **摘要**: 利用核磁共振技术解析重组人UNR蛋白的冷休克结构域(CSD)，阐明其多结构域协同作用介导RNA结合及基因表达调控的分子基础。
3. **"UNR is required for translational control of certain mRNAs during embryonic development"**
- **作者**: *Mihailovich M, Thermann R, Grohbauer S, et al.*
- **摘要**: 通过重组UNR蛋白功能实验，发现其在胚胎发育中特异性调控靶标mRNA的翻译效率，可能与癌症中UNR异常表达相关。
4. **"Cold shock domain proteins and the regulation of gene expression in human cancers"**
- **作者**: *Chang Z, Chaga O, Jonsson BH, et al.*
- **摘要**: 综述UNR(CSDC1)蛋白的重组表达及功能，强调其在肿瘤发生中通过结合致癌mRNA调控翻译及细胞增殖的作用。
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**注**：以上条目参考了UNR蛋白在RNA调控、结构生物学及癌症领域的核心研究，涉及重组蛋白的制备与功能验证。如需完整文献信息，可进一步提供DOI或数据库链接。
Recombinant human UNR (Upstream of N-Ras) protein, also known as CSDE1 (Cold Shock Domain Containing E1), is a multifunctional RNA-binding protein involved in post-transcriptional gene regulation. Discovered upstream of the N-Ras oncogene, UNR contains five cold shock domains (CSDs) that enable RNA binding, facilitating interactions with AU-rich elements (AREs) in mRNA untranslated regions (UTRs). It plays roles in mRNA stability, translation, and localization, influencing processes like cell proliferation, differentiation, and stress responses. UNR is implicated in cancer, where dysregulated expression correlates with tumor progression and metastasis. It also modulates viral replication by interacting with pathogen RNA, such as in HIV and hepatitis C virus.
Recombinant UNR is typically produced using bacterial (e.g., *E. coli*) or eukaryotic expression systems, followed by affinity purification. Researchers employ it to study RNA-protein interactions, translation mechanisms, and cellular pathways in vitro. Its modular domains and regulatory versatility make it a tool for dissecting RNA biology and developing therapeutic strategies targeting RNA-driven diseases. Ongoing research explores UNR's potential as a biomarker or drug target, particularly in oncology and virology.
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