| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNKL |
| Uniprot No | Q9H9P5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680 aa |
| 活性数据 | MPSVSKAAAA ALSGSPPQTE KPTHYRYLKE FRTEQCPLFS QHKCAQHRPF TCFHWHFLNQ RRRRPLRRRD GTFNYSPDVY CSKYNEATGV CPDGDECPYL HRTTGDTERK YHLRYYKTGT CIHETDARGH CVKNGLHCAF AHGPLDLRPP VCDVRELQAQ EALQNGQLGG GEGVPDLQPG VLASQAMIEK ILSEDPRWQD ANFVLGSYKT EQCPKPPRLC RQGYACPHYH NSRDRRRNPR RFQYRSTPCP SVKHGDEWGE PSRCDGGDGC QYCHSRTEQQ FHPESTKCND MRQTGYCPRG PFCAFAHVEK SLGMVNEWGC HDLHLTSPSS TGSGQPGNAK RRDSPAEGGP RGSEQDSKQN HLAVFAAVHP PAPSVSSSVA SSLASSAGSG SSSPTALPAP PARALPLGPA SSTVEAVLGS ALDLHLSNVN IASLEKDLEE QDGHDLGAAG PRSLAGSAPV AIPGSLPRAP SLHSPSSAST SPLGSLSQPL PGPVGSSAMT PPQQPPPLRS EPGTLGSAAS SYSPLGLNGV PGSIWDFVSG SFSPSPSPIL SAGPPSSSSA SPNGAELARV RRQLDEAKRK IRQWEESWQQ VKQVCDAWQR EAQEAKERAR VADSDRQLAL QKKEEVEAQV IFQLRAKQCV ACRERAHGAV LRPCQHHILC EPCAATAPEC PYCKGQPLQW |
| 分子量 | 73.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UNKL蛋白的虚构参考文献示例，格式为文献名称、作者及摘要概括：
1. **《UNKL蛋白在细胞周期调控中的功能研究》**
*作者：Smith J, et al.*
摘要：本研究揭示了UNKL通过结合特定mRNA调控G1/S期转换，其缺失导致细胞周期阻滞，提示其在增殖性疾病中的潜在作用。
2. **《UNKL作为RNA结合蛋白的结构与机制解析》**
*作者：Li X, et al.*
摘要：通过晶体学分析，发现UNKL的锌指结构域介导RNA相互作用，并参与胚胎发育中神经嵴细胞的迁移调控。
3. **《UNKL在急性髓系白血病中的异常表达及临床意义》**
*作者：Garcia R, et al.*
摘要：UNKL在白血病患者中高表达，通过激活Wnt/β-catenin通路促进癌细胞侵袭，可能成为治疗靶点。
4. **《UNKL基因敲除小鼠模型揭示其在心脏发育中的必要性》**
*作者：Tanaka M, et al.*
摘要：UNKL缺失导致小鼠胚胎心脏畸形，证实其通过调控心肌细胞分化相关基因剪切参与心脏形态发生。
注：以上为模拟文献，实际研究中请通过学术数据库检索真实论文。
**Background of Recombinant Human UNKL Protein**
The UNKL (Unkempt-like) protein, a member of the "Unkempt" family, is a zinc finger-containing protein encoded by the *UNKL* gene in humans. Although its biological functions remain partially characterized, UNKL shares structural homology with UNK, which is implicated in regulating transcription, cell differentiation, and developmental processes. UNKL contains conserved C3H1-type zinc finger motifs, suggesting roles in nucleic acid binding or protein interactions, though specific molecular mechanisms are underexplored.
Expressed in various tissues, including the brain, heart, and immune cells, UNKL’s expression patterns hint at potential roles in tissue-specific signaling or homeostasis. Recent studies link *UNKL* dysregulation to diseases such as cancer and neurodevelopmental disorders, though causal relationships remain unclear. Recombinant human UNKL protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies, antibody development, and mechanistic explorations. Its purification often involves affinity tagging (e.g., His-tag) to facilitate isolation.
Current research focuses on deciphering UNKL’s interaction networks, post-translational modifications, and regulatory pathways. Challenges include elucidating its precise physiological roles and validating its therapeutic relevance. Further characterization of recombinant UNKL may advance understanding of its contributions to cellular processes and disease, positioning it as a potential biomarker or drug target.
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