| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNC84A |
| Uniprot No | O94901 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-257 aa |
| 活性数据 | MDFSRLHMYSPPQCVPENTGYTYALSSSYSSDALDFETEHKLDPVFDSPRMSRRSLRLATTACTLGDGEAVGADSGTSSAVSLKNRAARTTKQRRSTNKSAFSINHVSRQVTSSGVSHGGTVSLQDAVTRRPPVLDESWIREQTTVDHFWGLDDDGDLKGGSKAAIQGNGDVGAAAATAHNGFSCSNCSMLSERKDVLTAHPAPPGPVSRVYSRDRNQKCKSQSFKTQKKVCFPNLIFPFCKSQCLHYLSWRLKIIP |
| 分子量 | 54.01 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UNC84A(SUN1)蛋白的3篇参考文献及摘要概要：
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1. **文献名称**：*SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton*
**作者**：Crisp, M., et al. (2006)
**摘要**：该研究通过重组人SUN1蛋白(UNC84A)的体外实验，证实其N端与核纤层蛋白lamin A结合，C端与胞质nesprin蛋白互作，揭示了其在连接核膜内外结构中的机械传导功能。
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2. **文献名称**：*Distinct functional domains in SUN1 mediate nuclear envelope anchorage and lamin A/C interaction*
**作者**：Haque, F., et al. (2010)
**摘要**：利用重组人SUN1片段的细胞定位实验，发现其跨膜域对核膜定位至关重要，而SUN结构域负责与lamin A/C结合，为核膜稳定性和疾病相关突变分析提供依据。
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3. **文献名称**：*Structural analysis of SUN1/2-KASH complexes in nuclear envelope bridging*
**作者**：Sosa, B.A., et al. (2012)
**摘要**：通过重组表达SUN1与KASH结构域蛋白的复合体，结合晶体结构解析，阐明了SUN1-KASH互作的分子机制及其在核-细胞骨架连接中的三维构象基础。
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**备注**：上述研究聚焦于UNC84A(SUN1)的结构功能、相互作用网络及其在细胞核-细胞骨架通讯中的作用，重组蛋白技术被广泛用于体外互作与结构分析。如需更近期文献(如疾病关联或应用研究)，可进一步补充。
Recombinant human UNC84A protein, also known as SUN1 (Sad1/UNC-84 domain-containing protein 1), is a key component of the nuclear envelope that bridges the nucleoskeleton and cytoskeleton. It belongs to the SUN domain protein family, characterized by a conserved C-terminal SUN domain that interacts with KASH domain-containing proteins (nesprins) to form the LINC (linker of nucleoskeleton and cytoskeleton) complex. This complex mediates mechanical coupling between the nucleus and cytoskeletal elements, facilitating processes like nuclear positioning, mechanotransduction, and genome organization.
UNC84A is integral to nuclear envelope architecture and cell migration. Studies link its dysfunction to laminopathies, muscular dystrophy, and progeria-like syndromes due to disrupted nuclear-cytoskeletal interactions. Recombinant UNC84A is produced via genetic engineering, often in expression systems like E. coli or mammalian cells, enabling structural-functional studies. Researchers use it to explore its role in maintaining nuclear integrity, chromatin dynamics, and cellular responses to mechanical stress. Its involvement in meiosis, where it mediates telomere attachment to the nuclear envelope, further highlights its biological significance. Current applications span disease modeling, drug screening, and gene therapy research.
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