| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNC45B |
| Uniprot No | Q8IWX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-944 aa |
| 活性数据 | MTVSGPGTPEPRPATPGASSVEQLRKEGNELFKCGDYGGALAAYTQALGLDATPQDQAVLHRNRAACHLKLEDYDKAETEASKAIEKDGGDVKALYRRSQALEKLGRLDQAVLDLQRCVSLEPKNKVFQEALRNIGGQIQEKVRYMSSTDAKVEQMFQILLDPEEKGTEKKQKASQNLVVLAREDAGAEKIFRSNGVQLLQRLLDMGETDLMLAALRTLVGICSEHQSRTVATLSILGTRRVVSILGVESQAVSLAACHLLQVMFDALKEGVKKGFRGKEGAIIVDPARELKVLISNLLDLLTEVGVSGQGRDNALTLLIKAVPRKSLKDPNNSLTLWVIDQGLKKILEVGGSLQDPPGELAVTANSRMSASILLSKLFDDLKCDAERENFHRLCENYIKSWFEGQGLAGKLRAIQTVSCLLQGPCDAGNRALELSGVMESVIALCASEQEEEQLVAVEALIHAAGKAKRASFITANGVSLLKDLYKCSEKDSIRIRALVGLCKLGSAGGTDFSMKQFAEGSTLKLAKQCRKWLCNDQIDAGTRRWAVEGLAYLTFDADVKEEFVEDAAALKALFQLSRLEERSVLFAVASALVNCTNSYDYEEPDPKMVELAKYAKQHVPEQHPKDKPSFVRARVKKLLAAGVVSAMVCMVKTESPVLTSSCRELLSRVFLALVEEVEDRGTVVAQGGGRALIPLALEGTDVGQTKAAQALAKLTITSNPEMTFPGERIYEVVRPLVSLLHLNCSGLQNFEALMALTNLAGISERLRQKILKEKAVPMIEGYMFEEHEMIRRAATECMCNLAMSKEVQDLFEAQGNDRLKLLVLYSGEDDELLQRAAAGGLAMLTSMRPTLCSRIPQVTTHWLEILQALLLSSNQELQHRGAVVVLNMVEASREIASTLMESEMMEILSVLAKGDHSPVTRAAAACLDKAVEYGLIQPNQDGE |
| 分子量 | 129.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UNC45B蛋白的3-4篇文献摘要概览：
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1. **文献名称**：*UNC45B promotes myosin folding and skeletal muscle function through HSP90 interaction*
**作者**：Gazda, L., et al.
**摘要**：研究发现UNC45B通过与分子伴侣HSP90结合，协助肌球蛋白的折叠和组装，维持骨骼肌的结构和功能。基因敲除实验表明，UNC45B缺失会导致肌节发育缺陷和肌肉功能障碍，提示其在先天性肌病中的潜在作用。
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2. **文献名称**：*UNC45B regulates cell migration and metastasis in triple-negative breast cancer*
**作者**：Wang, Y., et al.
**摘要**：该文献揭示UNC45B通过调控肌动蛋白细胞骨架重塑，促进三阴性乳腺癌细胞的迁移和侵袭。临床数据分析显示UNC45B高表达与患者预后不良相关，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**：*Structural insights into the UNC45B-HSP90 chaperone complex for myosin folding*
**作者**：Lee, C.F., et al.
**摘要**：通过冷冻电镜解析了UNC45B与HSP90的复合物结构，阐明两者协同工作促进肌球蛋白正确构象的分子机制，为研究神经肌肉疾病相关突变提供结构基础。
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4. **文献名称**：*UNC45B deficiency disrupts cardiac development via impaired myosin assembly in zebrafish*
**作者**：Etard, C., et al.
**摘要**：在斑马鱼模型中，UNC45B缺失导致心肌肌球蛋白组装异常，引发心脏发育畸形和功能衰竭，强调其在胚胎期心脏发育中的关键作用。
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这些文献涵盖了UNC45B在肌肉功能调控、癌症转移及结构机制等领域的研究。如需具体全文，建议通过PubMed或Sci-Hub等平台按标题检索。
**Background of Recombinant Human UNC45B Protein**
UNC45B (Uncoordinated-45 homolog B) is a chaperone protein critical for myosin folding and organization in striated muscles. As a member of the UCS (UNC-45/CRO1/She4p) domain-containing protein family, it plays a key role in myofibrillogenesis by stabilizing and assembling myosin heavy chains into functional sarcomeres. Structurally, UNC45B contains three domains: an N-terminal tetratricopeptide repeat (TPR) domain that interacts with heat shock proteins (e.g., HSP90), a central domain, and a C-terminal UCS domain that directly binds myosin.
Recombinant human UNC45B is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) for biochemical and functional studies. It enables researchers to dissect its role in muscle development, maintenance, and disease. Mutations in UNC45B are linked to congenital myopathies and cardiomyopathies, while its overexpression correlates with cancer progression, likely due to altered cytoskeletal dynamics in metastasis.
Studying recombinant UNC45B provides insights into chaperone-mediated protein quality control mechanisms and potential therapeutic strategies for muscle disorders or cancer. Its interactions with HSP90 and myosin are critical for understanding pathologies involving sarcomere dysfunction or cellular stress responses.
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