| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNC50 |
| Uniprot No | Q53HI1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-259 aa |
| 活性数据 | MLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQMDFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFVLDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVHLNAFYPLLVILHFIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSALPFLKNTVILLYPFAPLILLYGLSLALGWNFTHTLCSFYKYRVK |
| 分子量 | 56.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人UNC50蛋白相关的模拟参考文献及摘要概括(注：文献名为虚构示例，实际文献需根据数据库查询)：
1. **文献名称**: "Characterization of Human UNC50 as a Novel RNA Splicing Regulator"
**作者**: Li, X. et al.
**摘要**: 研究报道了重组人UNC50蛋白的克隆与原核表达，并证实其通过调控剪接体组装参与pre-mRNA加工，影响靶基因选择性剪接。
2. **文献名称**: "UNC50 Interacts with SMN1 and Modifies Spinal Muscular Atrophy Pathology"
**作者**: Zhang, Y. et al.
**摘要**: 发现重组UNC50蛋白与运动神经元存活蛋白SMN1直接互作，可能通过稳定SMN复合物缓解脊髓性肌萎缩症模型的表型。
3. **文献名称**: "Structural Insights into UNC50's Role in Intracellular Trafficking"
**作者**: Wang, T. et al.
**摘要**: 解析了重组UNC50的晶体结构，揭示了其通过C端结构域介导高尔基体到内质网的囊泡运输调控机制。
**提示**：实际文献需通过PubMed或Google Scholar以"UNC50"或"uncoordinated 50 homolog"为关键词检索，建议补充研究年份或DOI获取全文。
**Background of Recombinant Human UNC50 Protein**
UNC50 is a conserved eukaryotic protein initially characterized in *Caenorhabditis elegans*, where it regulates trafficking of acetylcholine receptors. In humans, UNC50 (Uncoordinated-50 homolog) is a 434-amino acid protein localized to the endoplasmic reticulum (ER) and Golgi apparatus. It plays critical roles in membrane trafficking, protein sorting, and maintenance of ER homeostasis. Structurally, UNC50 contains a conserved DUF1732 domain, though its exact molecular functions remain under investigation.
Studies suggest UNC50 interacts with components of the vesicular transport machinery, including COPI and COPII complexes, influencing cargo protein exit from the ER. Dysregulation of UNC50 has been linked to pathologies such as cancer and neurodegenerative disorders. For instance, reduced UNC50 expression correlates with ER stress and apoptosis in neurons, implicating it in diseases like Alzheimer’s. In cancer, UNC50 may modulate cell proliferation and drug resistance pathways.
Recombinant human UNC50 protein is typically produced in *E. coli* or mammalian expression systems, enabling biochemical studies (e.g., binding assays) and functional analyses (e.g., CRISPR-mediated knockdown models). Its purification often involves affinity chromatography, yielding high-purity samples for structural or cell-based research. Current research focuses on elucidating UNC50’s role in organelle crosstalk, autophagy, and as a potential therapeutic target for ER stress-related diseases.
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