| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNC84B |
| Uniprot No | Q9UH99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-717 aa |
| 活性数据 | MSRRSQRLTRYSQGDDDGSSSSGGSSVAGSQSTLFKDSPLRTLKRKSSNMKRLSPAPQLGPSSDAHTSYYSESLVHESWFPPRSSLEELHGDANWGEDLRVRRRRGTGGSESSRASGLVGRKATEDFLGSSSGYSSEDDYVGYSDVDQQSSSSRLRSAVSRAGSLLWMVATSPGRLFRLLYWWAGTTWYRLTTAASLLDVFVLTRRFSSLKTFLWFLLPLLLLTCLTYGAWYFYPYGLQTFHPALVSWWAAKDSRRPDEGWEARDSSPHFQAEQRVMSRVHSLERRLEALAAEFSSNWQKEAMRLERLELRQGAPGQGGGGGLSHEDTLALLEGLVSRREAALKEDFRRETAARIQEELSALRAEHQQDSEDLFKKIVRASQESEARIQQLKSEWQSMTQESFQESSVKELRRLEDQLAGLQQELAALALKQSSVAEEVGLLPQQIQAVRDDVESQFPAWISQFLARGGGGRVGLLQREEMQAQLRELESKILTHVAEMQGKSAREAAASLSLTLQKEGVIGVTEEQVHHIVKQALQRYSEDRIGLADYALESGGASVISTRCSETYETKTALLSLFGIPLWYHSQSPRVILQPDVHPGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQEGTLLGKFTYDQDGEPIQTFHFQAPTMATYQVVELRILTNWGHPEYTCIYRFRVHGEPAH |
| 分子量 | 105.27 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UNC84B(SUN2)蛋白的3篇代表性文献，包含作者、文献名称和摘要概括：
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1. **文献名称**: *Structural and functional analysis of the SUN-KASH nuclear envelope complex*
**作者**: Sosa BA, Rothballer A, Kutay U, Schwartz TU
**摘要**: 本文解析了SUN2蛋白与KASH结构域蛋白的复合物晶体结构，揭示了其介导核膜与细胞骨架连接的分子机制。研究利用重组人SUN2蛋白进行体外结合实验，证实其与Nesprin蛋白相互作用的关键结构域。
2. **文献名称**: *The LINC complex is essential for cytoplasmic nuclear anchoring in migrating fibroblasts*
**作者**: Lu W, Schneider M, Neumann S, et al.
**摘要**: 研究通过重组表达SUN2蛋白，证明其在LINC复合体中的核心作用，调控细胞迁移过程中细胞核的机械锚定。实验显示SUN2缺失导致核膜与微管的连接受损。
3. **文献名称**: *SUN1/2 and Syne/Nesprin-1/2 complexes connect centrosome to the nucleus*
**作者**: Haque F, Mazzeo D, Patel JT, et al.
**摘要**: 研究发现重组SUN2蛋白与Syne/Nesprin家族蛋白形成复合物，将中心体与核膜相连。实验表明SUN2的N端结构域对维持核形态和染色体空间分布至关重要。
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**注**：人源UNC84B通常对应SUN2蛋白(与线虫Unc-84同源)，相关研究集中于其作为LINC复合体核心组分的结构和功能，涉及核膜连接、机械传导及细胞迁移等领域。如需具体文献来源，可基于上述信息在PubMed/Google Scholar进一步检索。
Recombinant human UNC84B (also known as SUN2) is a protein belonging to the Sad1/UNC-84 (SUN) domain-containing family, which plays critical roles in nuclear envelope organization and nucleocytoskeletal connections. UNC84B is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complexes, bridging the nuclear envelope by interacting with KASH domain proteins in the outer nuclear membrane. This interaction mediates mechanical coupling between the nuclear lamina and the cytoskeleton, facilitating processes like nuclear positioning, cell migration, and mechanotransduction.
UNC84B is anchored in the inner nuclear membrane, where its SUN domain binds to KASH-domain partners, while its N-terminal region interacts with chromatin and nuclear lamina components. Studies highlight its involvement in maintaining nuclear stability, regulating gene expression, and responding to mechanical stimuli. Dysregulation of UNC84B has been linked to cellular abnormalities, including defective nuclear morphology and impaired cell differentiation, with implications in laminopathies and cancers.
Recombinant versions of UNC84B are produced via heterologous expression systems (e.g., bacteria, mammalian cells) for structural and functional studies, enabling researchers to dissect its molecular interactions, post-translational modifications, and role in diseases. Its recombinant form is essential for in vitro assays, structural biology (e.g., crystallography), and exploring therapeutic targets for nuclear envelope-related disorders.
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