| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ULK3 |
| Uniprot No | Q6PHR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472 aa |
| 活性数据 | MAGPGWGPPR LDGFILTERL GSGTYATVYK AYAKKDTREV VAIKCVAKKS LNKASVENLL TEIEILKGIR HPHIVQLKDF QWDSDNIYLI MEFCAGGDLS RFIHTRRILP EKVARVFMQQ LASALQFLHE RNISHLDLKP QNILLSSLEK PHLKLADFGF AQHMSPWDEK HVLRGSPLYM APEMVCQRQY DARVDLWSMG VILYEALFGQ PPFASRSFSE LEEKIRSNRV IELPLRPLLS RDCRDLLQRL LERDPSRRIS FQDFFAHPWV DLEHMPSGES LGRATALVVQ AVKKDQEGDS AAALSLYCKA LDFFVPALHY EVDAQRKEAI KAKVGQYVSR AEELKAIVSS SNQALLRQGT SARDLLREMA RDKPRLLAAL EVASAAMAKE EAAGGEQDAL DLYQHSLGEL LLLLAAEPPG RRRELLHTEV QNLMARAEYL KEQVKMRESR WEADTLDKEG LSESVRSSCT LQ |
| 分子量 | 53.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ULK3蛋白的3条参考文献及其摘要概括：
1. **文献名称**: *ULK3 regulates the catabolic process of chondrocyte metabolism via inhibition of the Wnt/β-catenin pathway*
**作者**: Li, X., Li, Y., Jin, J. 等
**摘要**: 该研究报道ULK3通过抑制Wnt/β-catenin通路调控软骨细胞的代谢分解过程，影响骨关节炎的发展。ULK3表达降低与软骨退化相关，可能成为治疗靶点。
2. **文献名称**: *AMPK phosphorylates ULK3 and inhibits its kinase activity to regulate focal adhesion dynamics*
**作者**: Chan, E.Y.W., Tooze, S.A.
**摘要**: 研究发现AMPK在能量应激状态下磷酸化ULK3.抑制其激酶活性，进而调节细胞黏着斑的动态变化，揭示ULK3在细胞代谢与结构重塑中的新功能。
3. **文献名称**: *The autophagy-related kinase ULK3 interacts with Rab5 to regulate early endosomal trafficking*
**作者**: Eiyama, A., Yoshimori, T.
**摘要**: 本文揭示ULK3通过与Rab5蛋白互作调控早期内体运输，影响自噬小体的形成，表明ULK3在自噬膜运输中的直接作用，扩展了其在细胞器动态中的角色。
注：上述文献为模拟示例，实际研究中建议通过学术数据库(如PubMed)以“ULK3”或“Unc-51 like kinase 3”为关键词检索最新文献。
Here’s a concise description of recombinant human ULK3 protein in English, adhering to your requirements:
Recombinant human ULK3 (Unc-51 like kinase 3) protein is a serine/threonine kinase belonging to the ULK/ATG1 family, known for its role in regulating autophagy and cellular stress responses. ULK3 shares structural homology with ULK1 and ULK2. featuring a conserved N-terminal kinase domain and a C-terminal regulatory domain. Unlike its family members, ULK3 exhibits distinct functional characteristics, acting as a downstream effector of signaling pathways such as TGF-β and Sonic Hedgehog (SHH). It modulates autophagy initiation by interacting with mTOR and AMPK pathways, though its mechanisms are less defined than ULK1/2. Recombinant ULK3 is typically produced in bacterial or mammalian expression systems, enabling studies on kinase activity, substrate interactions, and pathway crosstalk. Its applications include deciphering autophagy dynamics, cancer biology (notably in glioblastoma and breast cancer), and neurodegenerative disease research. ULK3’s dual role in promoting autophagy and cell cycle arrest makes it a potential therapeutic target, driving interest in recombinant forms for drug screening and mechanistic studies. Current research focuses on clarifying its tissue-specific functions and post-translational regulation.
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