| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UHRF1 |
| Uniprot No | A0JNW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-806 aa |
| 活性数据 | MGVFAVPPLSSDTMWIQVRTMDGRQTHTVDSLSRLTKVEELRRKIQELFHVEPGLQRLFYRGKQMEDGHTLFDYEVRLNDTIQLLVRQSLVLPHSTKERDSELSDTDSGCCLGQSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGAWFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGPSCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLSSVPSEDEWYCPECRNDASEVVLAGERLRESKKKAKMASATSSSQRDWGKGMACVGRTKECTIVPSNHYGPIPGIPVGTMWRFRVQVSESGVHRPHVAGIHGRSNDGAYSLVLAGGYEDDVDHGNFFTYTGSGGRDLSGNKRTAEQSCDQKLTNTNRALALNCFAPINDQEGAEAKDWRSGKPVRVVRNVKGGKNSKYAPAEGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDDEPGPWTKEGKDRIKKLGLTMQYPEGYLEALANREREKENSKREEEEQQEGGFASPRTGKGKWKRKSAGGGPSRAGSPRRTSKKTKVEPYSLTAQQSSLIREDKSNAKLWNEVLASLKDRPASGSPFQLFLSKVEETFQCICCQELVFRPITTVCQHNVCKDCLDRSFRAQVFSCPACRYDLGRSYAMQVNQPLQTVLNQLFPGYGNGR |
| 分子量 | 115.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UHRF1蛋白的3篇参考文献及其简要摘要：
1. **"Structural basis for recognition of hemi-methylated DNA by the SRA domain of UHRF1"**
- **作者**: Arita, K. et al.
- **摘要**: 本文解析了UHRF1蛋白中SRA结构域的晶体结构，揭示其通过特异性结合半甲基化DNA的机制，并阐明了其在DNA甲基化遗传维持中的关键作用。
2. **"UHRF1 plays a role in maintaining DNA methylation in mammalian cells"**
- **作者**: Bostick, M. et al.
- **摘要**: 研究通过重组UHRF1蛋白的功能实验，证明其通过与DNA甲基转移酶(DNMT1)相互作用，在细胞分裂过程中确保DNA甲基化模式的准确复制。
3. **"Recognition of multivalent histone states associated with heterochromatin by UHRF1"**
- **作者**: Rajakumara, E. et al.
- **摘要**: 该文献利用重组UHRF1蛋白研究其与多种组蛋白修饰(如H3K9me3和H3K18ac)的多价结合模式，揭示了其在异染色质形成和表观遗传沉默中的调控机制。
4. **"UHRF1 depletion and HDAC inhibition synergistically induce apoptosis in acute myeloid leukemia"**
- **作者**: Liang, Q. et al.
- **摘要**: 通过重组UHRF1蛋白及基因编辑技术，研究发现抑制UHRF1可增强组蛋白去乙酰化酶(HDAC)抑制剂的抗白血病效果，为靶向表观遗传治疗的策略提供了依据。
以上文献涵盖了UHRF1的结构解析、表观遗传功能机制及治疗潜力等方向。
Ubiquitin-like with PHD and RING Finger Domains 1 (UHRF1), also known as NP95 or ICBP90. is a multidomain epigenetic regulator crucial for maintaining DNA methylation patterns during cell division. By bridging DNA methylation and histone modifications, it ensures the faithful inheritance of epigenetic information. Structurally, UHRF1 contains a ubiquitin-like domain (UBL), tandem Tudor domain (TTD), plant homeodomain (PHD), SET- and RING-associated (SRA) domain, and a RING-type E3 ubiquitin ligase domain. The SRA domain recognizes hemi-methylated DNA through 5-methylcytosine, while its TTD and PHD domains interact with unmodified histone H3 tails, preferentially binding to H3K9me0 and H3K4me0. This dual recognition mechanism couples DNA replication with the restoration of repressive histone marks. UHRF1’s RING domain recruits DNMT1 to replication foci, facilitating methylation of newly synthesized DNA. Additionally, it exhibits E3 ubiquitin ligase activity toward histone H3. promoting chromatin condensation. Dysregulation of UHRF1 is strongly linked to cancer, as its overexpression in malignancies disrupts tumor suppressor gene silencing via aberrant DNA hypermethylation. Recombinant human UHRF1 protein, typically expressed in E. coli or insect cells, serves as a key tool for studying DNA methylation maintenance, chromatin remodeling, and drug discovery targeting epigenetic disorders. Current research focuses on its role in carcinogenesis, genome stability, and potential as a therapeutic biomarker.
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