| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UGT3A2 |
| Uniprot No | Q3SY77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523 aa |
| 活性数据 | MAGQRVLLLVGFLLPGVLLSEAAKILTISTVGGSHYLLMDRVSQILQDHGHNVTMLNHKRGPFMPDFKKEEKSYQVISWLAPEDHQREFKKSFDFFLEETLGGRGKFENLLNVLEYLALQCSHFLNRKDIMDSLKNENFDMVIVETFDYCPFLIAEKLGKPFVAILSTSFGSLEFGLPIPLSYVPVFRSLLTDHMDFWGRVKNFLMFFSFCRRQQHMQSTFDNTIKEHFTEGSRPVLSHLLLKAELWFINSDFAFDFARPLLPNTVYVGGLMEKPIKPVPQDLENFIAKFGDSGFVLVTLGSMVNTCQNPEIFKEMNNAFAHLPQGVIWKCQCSHWPKDVHLAANVKIVDWLPQSDLLAHPSIRLFVTHGGQNSIMEAIQHGVPMVGIPLFGDQPENMVRVEAKKFGVSIQLKKLKAETLALKMKQIMEDKRYKSAAVAASVILRSHPLSPTQRLVGWIDHVLQTGGATHLKPYVFQQPWHEQYLLDVFVFLLGLTLGTLWLCGKLLGMAVWWLRGARKVKET |
| 分子量 | 85.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UGT3A2蛋白的3条虚构参考文献(注：文献及内容为示例，非真实数据)：
1. **"Functional characterization of recombinant human UGT3A2 in steroid glucuronidation"**
*Authors: Smith J, Lee R, Tanaka A*
**摘要**：研究通过重组表达UGT3A2蛋白，分析其对多种甾体类化合物的葡萄糖醛酸化活性，发现其对脱氢表雄酮(DHEA)代谢有特异性，揭示了UGT3A2在激素调控中的潜在作用。
2. **"Substrate specificity and tissue distribution of UGT3A2: Insights from recombinant enzyme assays"**
*Authors: Chen L, Park H, Gupta S*
**摘要**：通过体外酶学实验系统评估重组UGT3A2的底物偏好性，发现其对某些胆汁酸和酚类化合物具有显著催化活性，并通过RNA-seq数据推测其在肠道和肾脏中可能发挥代谢功能。
3. **"Structural analysis of human UGT3A2 reveals unique substrate-binding motifs"**
*Authors: Müller F, Zhou Y, Davies E*
**摘要**：利用重组UGT3A2蛋白进行晶体结构解析，鉴定出其底物结合域中的特定氨基酸残基(如Arg156和Glu289)对催化活性的关键影响，为UGT3A2功能差异提供分子机制解释。
**注**：上述文献及内容均为示例，实际研究中需通过数据库(如PubMed)检索真实文献。UGT3A2相关研究较少，可扩展搜索UGT3家族或糖基转移酶相关综述获取更多信息。
The recombinant human UGT3A2 protein is a member of the uridine diphosphate (UDP)-glucuronosyltransferase (UGT) enzyme family, which plays a critical role in phase II metabolism by catalyzing the glucuronidation of endogenous and exogenous compounds. This process enhances the solubility of substrates, facilitating their biliary or renal excretion. UGT3A2. encoded by the UGT3A2 gene, is a less-characterized isoform compared to the well-studied UGT1A and UGT2B subfamilies. It is primarily expressed in extrahepatic tissues, such as the brain, skin, and gastrointestinal tract, suggesting tissue-specific metabolic functions. Structurally, UGT3A2 contains a conserved C-terminal catalytic domain for UDP-glucuronic acid binding but differs in substrate recognition due to its unique N-terminal domain. Recombinant UGT3A2 protein, typically produced via heterologous expression systems like mammalian or insect cells, is used to investigate its substrate specificity, enzymatic kinetics, and role in detoxification pathways. Research highlights its potential involvement in metabolizing neurosteroids, bile acids, and certain xenobiotics, contributing to interindividual variability in drug metabolism and disease susceptibility. Despite progress, its physiological relevance and regulatory mechanisms remain understudied, necessitating further exploration to clarify its contributions to human biochemistry and therapeutic applications.
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