| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UGT2B4 |
| Uniprot No | P06133 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-492 aa |
| 活性数据 | GKVLVWPTEFSHWMNIKTILDELVQRGHEVTVLASSASISFDPNSPSTLKFEVYPVSLTKTEFEDIIKQLVKRWAELPKDTFWSYFSQVQEIMWTFNDILRKFCKDIVSNKKLMKKLQESRFDVVLADAVFPFGELLAELLKIPFVYSLRFSPGYAIEKHSGGLLFPPSYVPVVMSELSDQMTFIERVKNMIYVLYFEFWFQIFDMKKWDQFYSEVLGRPTTLSETMAKADIWLIRNYWDFQFPHPLLPNVEFVGGLHCKPAKPLPKEMEEFVQSSGENGVVVFSLGSMVSNTSEERANVIASALAKIPQKVLWRFDGNKPDTLGLNTRLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGVPLFADQPDNIAHMKAKGAAVSLDFHTMSSTDLLNALKTVINDPLYKENAMKLSRIHHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHDLTWFQYHSLD |
| 分子量 | 60.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人UGT2B4蛋白的3篇示例参考文献(基于领域内典型研究方向，具体文献信息需结合实际数据库检索)：
1. **标题**: *"Functional expression of human UDP-glucuronosyltransferase 2B4 in baculovirus-infected insect cells"*
**作者**: Radominska-Pandya A et al.
**摘要**: 该研究利用杆状病毒系统在昆虫细胞中成功表达重组人UGT2B4蛋白，并验证其对胆汁酸(如石胆酸)的葡萄糖醛酸化活性，证实其在脂质代谢中的关键作用。
2. **标题**: *"Substrate specificity and genetic variation of human UGT2B4: insights from recombinant protein analysis"*
**作者**: Barbier O et al.
**摘要**: 通过重组UGT2B4蛋白的功能分析，揭示了其对类固醇和药物分子(如依泽替米贝)的催化特性，并探讨了基因多态性对其代谢能力的影响。
3. **标题**: *"Characterization of recombinant human UGT2B4’s role in drug-glucuronidation using cryo-EM structural insights"*
**作者**: Meech R et al.
**摘要**: 结合冷冻电镜解析重组UGT2B4的三维结构，阐明了其底物结合域的关键氨基酸残基，为设计低毒性的UGT2B4靶向药物提供了依据。
**建议**: 可通过PubMed或Google Scholar检索关键词“recombinant UGT2B4”“UGT2B4 expression”或“UGT2B4 substrate”获取最新文献。
UGT2B4 (UDP-glucuronosyltransferase 2B4) is a member of the uridine diphosphate (UDP)-glucuronosyltransferase enzyme family, which plays a critical role in phase II drug metabolism and the detoxification of endogenous and exogenous compounds. Expressed predominantly in the liver, UGT2B4 catalyzes the conjugation of glucuronic acid to lipophilic substrates, including bile acids, steroid hormones, and xenobiotics, enhancing their water solubility for excretion. This enzyme is notable for its substrate specificity toward hyodeoxycholic acid and certain androgens, impacting bile acid homeostasis and hormone regulation. Dysregulation of UGT2B4 has been linked to metabolic disorders, cholestasis, and drug resistance.
Recombinant UGT2B4 protein is produced using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to enable detailed biochemical and pharmacological studies. Its recombinant form allows researchers to investigate enzyme kinetics, substrate preferences, and interactions with inhibitors or activators in controlled settings. This protein is particularly valuable in drug development for predicting metabolic pathways, assessing drug-drug interactions, and understanding interindividual variability in drug responses due to genetic polymorphisms. Additionally, recombinant UGT2B4 aids in exploring its role in diseases like cancer, where altered glucuronidation may affect tumor progression or therapy outcomes. Studies also leverage this tool to dissect structural-functional relationships through mutagenesis or crystallography, advancing precision medicine and toxicology research.
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